Background: Venom peptides are a proven resource for identifying novel drugs, however
the process of identifying bioactive venom peptides is currently labor intensive, costly, and
rarely results in pharmaceutical success. As venom peptides are modulators of ion channels and
receptors their potential for manipulating cell signals in diseased states are unique and offer an untapped
resource for finding new medicines. Recent advances in -omic technologies, and microfluidic
biosensing systems have transformed how venom peptides are discovered and characterized.
Conclusion: This review will cover past, present and future approaches for screening venom peptides
for drug discovery and development. Specifically, we will highlight online high-resolution
microfluidic biosensing systems and new fluorescence detection methods that can be adapted to
expand the discovery and characterization of venom peptide drugs.