Lipases: Sources, Production, Purification, and Applications

Author(s): Naveen Patel*, Dhananjai Rai, Shivam, Shraddha Shahane, Umesh Mishra

Journal Name: Recent Patents on Biotechnology

Volume 13 , Issue 1 , 2019

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Graphical Abstract:


Background and Sources: Lipase enzyme is a naturally occurring enzyme found in the stomach and pancreatic juice. Its function is to digest fats and lipids, helping to maintain correct gallbladder function. Lipase is the one such widely used and versatile enzyme. These enzymes are obtained from animals, plants and as well as from several microorganisms and are sufficiently stable. These are considered as nature’s catalysts, but commercially, only microbial lipases are being used significantly.

Applications: They found enormous application in the industries of fat and oil processing, oleochemical industry, food industry, detergents, pulp and paper industry, detergents, environment management, tea processing, biosensors and cosmetics and perfumery. Various recent patents related to lipases have been revised in this review.

Conclusion: Lipases are very peculiar as they have the ability to hydrolyse fats into fatty acids and glycerols at the water-lipid interface and can reverse the reaction in non-aqueous media. This natural ability makes it the most widely used enzyme in various industrial applications. This article deals with the immense versatility of lipase enzymes along with the recent advancements done in the various fields related to their purification and mass production in industries.

Keywords: Lipases, enzyme, oleochemical industry, environment management, biosensors, catalysts.

Pliego J, Mateos JC, Rodriguez J, et al. Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate. Sensors 2015; 15: 2798-811.
Norus J. Building sustainable competitive advantage from knowledge in the region: the industrial enzymes industry. European Plann Stud 2006; 14: 681-96.
Dewan S. Enzymes in industrial applications: Global markets Market Research Repor t Wellesley. MA: BCC Research 2011.
Chandel AK, Rudravaram R, Rao LV, et al. Industrial enzymes in bioindustrial sector development: an Indian perspective. J Commer Biotechnol 2007; 13: 283-91.
Li S, Yang X, Yang S, et al. Technology prospecting on enzymes: application, marketing and engineering. Comput Struct Biotechnol J 2012; 2: e201209017.
Laachari F, El Bergad F, Sadiki M, et al. Higher tolerance of a novel lipase from Aspergillus flavus to the presence of free fatty acids at lipid/water interface. Afr J Biochem 2015; 9: 9-17.
Lee LP, Karbul HM, Citartan M, et al. Lipase-secreting Bacillus species in an oil-contaminated habitat: promising strains to alleviate oil pollution. BioMed Res Int 2015; 2015(1): 1-9.
Priji P, Unni KN, Sajith S, et al. Production, optimization, and partial purification of lipase from Pseudomonas sp. strain BUP6, a novel rumen bacterium characterized from Malabari goat. Biotechnol Appl Biochem 2015; 62: 71-8.
Nadeem U, Muhammad D, Muhammad S, et al. Screening identification and characterization of lipase producing soil bacteria from Upper Dir and Mardan Khyber Pakhtunkhwa, Pakistan. Int J Biosci 2015; 6: 49-55.
Chowdary G, Ramesh M, Prapulla S. Enzymic synthesis of isoamyl isovalerate using immobilized lipase from Rhizomucor miehei: a multivariate analysis. Process Biochem 2000; 36: 331-9.
Therisod M, Klibanov AM. Regioselective acylation of secondary hydroxyl groups in sugars catalyzed by lipases in organic solvents. J Am Chem Soc 1987; 109: 3977-81.
Rao P, Divakar S. Lipase catalyzed esterification of α-terpineol with various organic acids: application of the Plackett-Burman design. Process Biochem 2001; 36: 1125-8.
Harwood J. The versatility of lipases for industrial uses. Trends Biochem 1989; 14: 125-6.
Ananthi S, Ramasubburayan R, Palavesam A, et al. Optimization and purification of lipase through solid state fermentation by Bacillus cereus MSU as isolated from the gut of a marine fish Sardinella longiceps. Int J Pharma Bio Sci 2014; 5: 291-8.
Thakur V, Tewari R, Sharma R. Evaluation of production parameters for maximum lipase pro-duction by P. stutzeri MTCC 5618 and scale-up in bioreactor. Chin J Biol 2014; 2014: 1-14.
Iftikhar T, Niaz M, Ali EA, et al. Production process of extracellular lipases by Fusarium sp. using agricultural byproducts. Pak J Bot 2012; 44: 335-9.
Aires-Barros M, Taipa M, Cabral J, et al. Lipases-their structure, biochemistry and application. Cambridge, UK: Cambridge University 1994.
Alberghina L, Schmid R, Verger R. Lipases: Structure, Mechanism, and Genetic Engineering: Contributions to the CEC-GBF International Workshop. Braunschweig, Germany: Wiley- Blackwell 1991.
Hamosh M, Borgström B, Brockman HL. Lipases. Amsterdam: Elsevier 1984.
Malcata FX. Engineering of/with lipases: scope and strategies. Nato ASI 1996; 317: 1-16.
Rubin B, Dennes E. Lipases: Part A Biotechnology methods in enzymology. 1st ed. New York: Academic Press 1997.
Gunstone F, Harwood J, Padley F. The Lipid Handbook. 2nd ed. London, UK: Wiley 1994.
Ratledge C, Tan KH. Oils and fats: production, degradation and utilization by yeasts. In: Verachtert H, Ed. De Mot R Yeast Biotechnology and Biocatalysis. Newyork: Marcel Dekker Inc 1990; pp. 223-54.
Jaeger KE, Ransac S, Dijkstra BW, et al. Bacterial lipases. FEMS Microbiol Rev 1994; 15: 29-63.
Gilbert EJ. Pseudomonas lipases: biochemical pro-perties and molecular cloning. Enzyme Microb Technol 1993; 15: 634-45.
Gupta R, Gupta N, Rathi P. Bacterial lipases: an overview of production, purification and biochemical properties. Appl Microbiol Biotechnol 2004; 64: 763-81.
Singh AK, Mukhopadhyay M. Overview of fungal lipase: a review. Appl Biochem Biotechnol 2012; 166: 486-520.
Romo-Sánchez S, Alves-Baffi M, Arévalo-Villena M, et al. Yeast biodiversity from oleic ecosystems: study of their biotechnological properties. Food Microbiol 2010; 27: 487-92.
Lin S-F, Chiou C-M, Tsai Y-C. Effect of Triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111. Biotechnol Lett 1995; 17: 959-62.
Sztajer H, Maliszewska I, Wieczorek J. Production of exogenous lipases by bacteria, fungi, and actino-mycetes. Enzyme Microb Technol 1988; 10: 492-7.
Wang Y, Srivastava KC, Shen G-J, et al. Thermostable alkaline lipase from a newly isolated thermophilic Bacillus, strain A30-1 (ATCC 53841). J Ferment Bioeng 1995; 79: 433-8.
Jaeger KE, Ransac S, Dijkstra BW, et al. Bacterial lipases. FEMS Microbiol Rev 1994; 15: 29-63.
Eggert T, van Pouderoyen G, Dijkstra BW, et al. Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure. FEBS Lett 2001; 502: 89-92.
Arpigny JL, Jaeger K-E. Bacterial lipolytic enzymes: classification and properties. Biochem J 1999; 343: 177-83.
Castilla A, Panizza P, Rodríguez D, et al. A novel thermophilic and halophilic esterase from Janibacter sp. R02, the first member of a new lipase family (Family XVII). Enzyme Microb Technol 2017; 98: 86-95.
Masomian M, Rahman RNZRA, Salleh AB, et al. Analysis of comparative sequence and genomic data to verify phylogenetic relationship and explore a new subfamily of bacterial lipases. PLoS One 2016; 11: e0149851.
Rajendran A, Palanisamy A, Thangavelu V. Evaluation of medium components by Plackett-Burman statistical design for lipase production by Candida rugosa and kinetic modeling. Chin J Biotechnol 2008; 24: 436-44.
Burkert JFdM. Maugeri F, Rodrigues MI. Optimization of extracellular lipase production by Geotrichum sp. using factorial design. Bioresour Technol 2004; 91: 77-84.
Peters GH, Olsen O, Svendsen A, et al. Theoretical investigation of the dynamics of the active site lid in Rhizomucor miehei lipase. Biophys J 1996; 71: 119-29.
Chahinian H, Vanot G, Ibrik A, et al. Production of extracellular lipases by Penicillium cyclopium purification and characterization of a partial acylglycerol lipase. Biosci Biotechnol Biochem 2000; 64: 215-22.
Shukla P, Gupta K. Ecological screening for lipolytic molds and process optimization for lipase production from Rhizopus oryzae KG-5. J Appl Sci Environ Sanitat 2007; 2: 35-42.
Siddiqui KS, Cavicchioli R. Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharides. Extremophiles 2005; 9: 471-6.
Ibrahim CO, Hayashi M, Nagai S. Purification and some properties of a thermostable lipase from Humicola lanuginosa no. 3. Agric Biol Chem 1987; 51: 37-45.
Smerdon GR, Aves SJ, Walton EF. Production of human gastric lipase in the fission yeast Schizosaccharomyces pombe. Gene 1995; 165: 313-8.
Birari RB, Bhutani KK. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today 2007; 12: 879-89.
Siekevitz P, Palade GE. A cytochemical study on the pancreas of the Guinea Pig: V. In vivo incorporation of Leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Cell Biol 1960; 7: 619-30.
Liu Y, Chen D, Yan Y, et al. Biodiesel synthesis and conformation of lipase from Burkholderia cepacia in room temperature ionic liquids and organic solvents. Bioresour Technol 2011; 102: 10414-8.
Frenken LG, Bos JW, Visser C, et al. An accessory gene, lipB, required for the production of active Pseudomonas glumae lipase. Mol Microbiol 1993; 9: 579-89.
Khoramnia A, Ebrahimpour A, Beh BK, et al. Production of a solvent, detergent, and thermotolerant lipase by a newly isolated Acinetobacter sp. in submerged and solid-state fermentations. Biomed Res Int 2011; 2011:
Bajaj A, Lohan P, Jha PN, et al. Biodiesel production through lipase catalyzed transesterification: an overview. J Mol Catal, B Enzym 2010; 62: 9-14.
Nadeem H, Rashid HM, Siddique HM. Effect of Mg2+ and Al3+ ions on thermodynamic and physio-chemical properties of Aspergillus niger invertases. Protein Pept Lett 2015; 22: 743-9.
Yang W, He Y, Xu L, et al. A new extracellular thermo-solvent-stable lipase from Burkholderia ubonensis SL-4: Identification, characterization and application for biodiesel production. J Mol Catal, B Enzym 2016; 126: 76-89.
Unni KN, Priji P, Sajith S, et al. Pseudomonas aeruginosa strain BUP2, a novel bacterium inhabiting the rumen of Malabari goat, produces an efficient lipase. Biologia 2016; 71: 378-87.
Kirana S, Arshada Z, Nosheenb S, et al. Microbial lipases: production and applications: A Review. J Biotechnol Biomater 2016; 1: 7-20.
Chisti Y. Bioseparation and bioprocessing: a handbook Vch Verlag. Wiley 1998.
Jaeger K-E, Reetz MT. Microbial lipases form versatile tools for biotechnology. Trends Biotechnol 1998; 16: 396-403.
Ghosh P, Saxena R, Gupta R, et al. Microbial lipases: production and applications. Sci Prog 1996; 79(2): 119-57.
Zaks A, Klibanov AM. Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci 1985; 82: 3192-6.
Malcata FX, Reyes HR, Garcia HS, et al. Kinetics and mechanisms of reactions catalysed by immobilized lipases. Enzyme Microb Technol 1992; 14: 426-46.
Guit R, Kloosterman M, Meindersma G, et al. Lipase kinetics: Hydrolysis of triacetin by lipase from Candida cylindracea in a hollow-fiber membrane reactor. Biotechnol Bioeng 1991; 38: 727-32.
Nadeem H, Rashid MH, Siddique MH, et al. Microbial invertases: a review on kinetics, thermodynamics, physiochemical properties. Process Biochem 2015; 50: 1202-10.
Fullbrook P. Practical applied kinetics Industrial enzymology. 2nd ed. New York: Stockholm Press 1996.
Demir BS, Tükel SS. Purification and characteri-zation of lipase from Spirulina platensis. Mol Catal B Enzym 2010; 64: 123-8.
Gricajeva A, Bendikienė V, Kalėdienė L. Lipase of Bacillus stratosphericus L1: Cloning, expression and characterization. Int J Biol Macromol 2016; 92: 96-104.
Sivaramakrishnan R, Incharoensakdi A. Purification and characterization of solvent tolerant lipase from Bacillus sp. for methyl ester production from algal oil. J Biosci Bioeng 2016; 121: 517-22.
Benjamin S, Pandey A. Candida rugosa lipases: molecular biology and versatility in biotechnology. Yeast 1998; 14: 1069-87.
Laachari F, El Bergadi F, Sayari A, et al. Biochemical characterization of a new thermostable lipase from Bacillus pumilus strain. [Bacillus pumilus suşundan elde edilen yeni termostabil lipazın biyokimyasal karakterizasyonu]. Turk J Biochem 2015; 40: 8-14.
Sarac N, Ugur A, Boran R, et al. The use of boron compounds for stabilization of lipase from Pseudomonas aeruginosa ES3 for the detergent industry. J Surfactants Deterg 2015; 18: 275-85.
Ramakrishnan V, Goveas LC, Suralikerimath N, et al. Extraction and purification of lipase from Enterococcus faecium MTCC5695 by PEG/phosphate aqueous-two phase system (ATPS) and its biochemical characterization. Biocatal Agric Biotechnol 2016; 6: 19-27.
Lima V, Krieger N, Mitchell D, et al. Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents. Biochem Eng J 2004; 18: 65-71.
Tripathi R, Singh J. kumar Bharti R, et al. Isolation, purification and characterization of lipase from Microbacterium sp. and its application in biodiesel production. Energy Procedia 2014; 54: 518-29.
Yoo H-Y, Simkhada JR, Cho SS, et al. A novel alkaline lipase from Ralstonia with potential application in biodiesel production. Bioresour Technol 2011; 102: 6104-11.
Daoud L, Kamoun J, Ali MB, et al. Purification and biochemical characterization of a halotolerant Staphylococcus sp. extracellular lipase. Int J Biol Macromol 2013; 57: 232-7.
Kumar R, Sharma A, Kumar A, et al. Lipase from Bacillus pumilus RK31: production, purification and some properties. World Appl Sci J 2012; 16: 940-8.
Wang B, Wang A, Cao Z, et al. Characterization of a novel highly thermostable esterase from the Gram‐positive soil bacterium Streptomyces lividans TK64. Biotechnol Appl Biochem 2016; 63: 334-43.
Ji X, Chen G, Zhang Q, et al. Purification and characterization of an extracellular cold adapted alkaline lipase produced by psychrotrophic bacterium Yersinia enterocolitica strain KM1. J Basic Microbiol 2015; 55: 718-28.
Kazlauskas RJ, Bornscheuer UT. Biotechnology: Biotransformations I. 2nd ed. Vch Verlag: Wiley 1998.
Masse L, Kennedy KJ, Chou SP. The effect of an enzymatic pretreatment on the hydrolysis and size reduction of fat particles in slaughterhouse wastewater. J Chem Technol Biot 2001; 76: 629-35.
Takamoto T, Shirasaka H, Uyama H, et al. Lipase-catalyzed hydrolytic degradation of polyurethane in organic solvent. Chem Lett 2001; 30: 492-3.
Konkit M, Kim W. Activities of amylase, proteinase, and lipase enzymes from Lactococcus chungangensis and its application in dairy products. J Dairy Sci 2016; 99: 4999-5007.
Boonmahome P, Mongkolthanaruk W. Lipase-producing bacterium and its enzyme characterization. J Life SciTechnol 2013; 1: 125-31.
Adrio JL, Demain AL. Microbial enzymes: tools for biotechnological processes. Biomolecules 2014; 4: 117-39.
Sharma R, Chisti Y, Banerjee UC. Production, purification, characterization, and applications of lipases. Biotechnol Adv 2001; 19: 627-62.
Makhzoum A, Owusu-Apenten R, Knapp J. Purification and properties of lipase from Pseudomonas fluorescens strain 2D. Int Dairy J 1996; 6: 459-72.
Esteban-Torres M, Mancheño JM, de las Rivas B, et al. Characterization of a halotolerant lipase from the lactic acid bacteria Lactobacillus plantarum useful in food fermentations. LWT-Food Sci Technol 2015; 60: 246-52.
Laboret F, Perraud R. Lipase-catalyzed production of short-chain acids terpenyl esters of interest to the food industry. Appl Biochem Biotechnol 1999; 82: 185-98.
Sánchez M, Prim N, Rández–Gil F, et al. Engineering of baker’s yeasts, E. coli and Bacillus hosts for the production of Bacillus subtilis lipase A. Biotechnol Bioeng 2002; 78: 339-45.
Uhlig H. Industrial enzymes and their applications Vch Verlag. Wiley 1998.
Ahmed EH, Raghavendra T, Madamwar D. An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: application for ethyl caprylate synthesis. Bioresour Technol 2010; 101: 3628-34.
Gupta C, Prakash D, Gupta S. Biotechnological approach to microbial based perfumes and flavours. J Microbiol Exp 2015; 3: 221-30.
Jolly JF. Enzymatic methods of flavor modification. US9144249B2, 2015.
Kynclova E, Hartig A, Schalkhammer T. Oligonucleotide labelled lipase as a new sensitive hybridization probe and its use in bio-assays and biosensors. J Mol Recognit 1995; 8: 139-45.
Pandey A, Benjamin S, Soccol CR, et al. The realm of microbial lipases in biotechnology. Biotechnol Appl Biochem 1999; 29: 119-31.
Xu Y, Yu X. Bifunctional lipase mutant and methods of using same. US9890367B2, 2018.
Sangeetha R, Arulpandi I, Geetha A. Bacterial lipases as potential industrial biocatalysts: An overview. Res J Microbiol 2011; 6: 1-24.
Oki H, Naruse H, Minami H, et al. Process of obtaining rice-bran oil and defatted rice bran from fresh rice bran. US20150320069, 2015.
Rodrigues RC, Fernandez-Lafuente R. Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification. J Mol Catal, B Enzym 2010; 66: 15-32.
Lin JF, Lin Q, Li J, et al. Bacterial diversity of lipase-producing strains in different soils in southwest of China and characteristics of lipase. Afr J Microbiol Res 2012; 6: 3797-806.
Buchon L, Laurent P, Gounot A, et al. Temperature dependence of extracellular enzymes production by psychrotrophic and psychrophilic bacteria. Biotechnol Lett 2000; 22: 1577-81.
Selvam K, Vishnupriya B. Partial purification of lipase from Streptomyces variabilis NGP 3 and its application in bioremediation of waste water. Int J Pharm Sci Res 2013; 4: 4281.
Kanmani P, Aravind J, Kumaresan K. An insight into microbial lipases and their environmental facet. Int J Environ Sci Technol 2015; 12: 1147-62.
Kanmani P, Kumaresan K, Aravind J. Pretreatment of coconut mill effluent using celite-immobilized hydrolytic enzyme preparation from Staphylococcus pasteuri and its impact on anaerobic digestion. Biotechnol Prog 2015; 31: 1249-58.
Krajmalnik-Brown R, Halden RU, Wilson JW. Methods and systems for tracking bioremediation processes. US20150010945A1, 2015.
Sharma S, Kanwar SS. Organic solvent tolerant lipases and applications. Sci World J 2014; 2014: 1-15.
Ganguly S, Nandi S. Process optimization of lipase catalyzed synthesis of diesters in a packed bed reactor. Biochem Eng J 2015; 102: 2-5.
Kim J-H, Bhatia SK, Yoo D, et al. Lipase-catalyzed production of 6-o-cinnamoyl-sorbitol from d-sorbitol and cinnamic acid esters. Appl Biochem Biotechnol 2015; 176: 244-52.
Sonne DP, Vilsbøll T, Knop FK. Pancreatic amylase and lipase plasma concentrations are unaffected by increments in endogenous GLP-1 levels following liquid meal tests. Diabetes Care 2015; 38: 71-2.
Chaplin J, Gardiner N, Mitra R, et al. Process for preparing (-)-menthol and similar compounds. US20040058422A1, 2004.
Dong H, Gao S. Han Sp, et al. Purification and characterization of a Pseudomonas sp. lipase and its properties in non-aqueous media. Biotechnol Appl Biochem 1999; 30: 251-6.
Linko YY, Yan Wu X. Biocatalytic production of useful esters by two forms of lipase from Candida rugosa. J Chem Technol Biotechnol 1996; 65: 163-70.
Xie Y-C, Liu H-Z, Chen J-Y. Candida rugosa lipase catalyzed esterification of racemic ibuprofen with butanol: racemization of R-ibuprofen and chemical hydrolysis of S-ester formed. Biotechnol Lett 1998; 20: 455-8.
Guncheva M, Zhiryakova D. Catalytic properties and potential applications of Bacillus lipases. J Mol Catal, B Enzym 2011; 68: 1-21.
Horchani H, Aissa I, Ouertani S, et al. Staphylococcal lipases: biotechnological applications. J Mol Catal, B Enzym 2012; 76: 125-32.
Nagarajan S. New tools for exploring “old friends-microbial lipases”. Appl Biochem Biotechnol 2012; 168: 1163-96.
Svendsen A, Skjoet M, Yaver D, et al. Lipase variants for pharmaceutical use. US9539311B2, 2017.
Gillberg P-G, Graffner H, Starke I. Ibat inhibitors for the treatment of liver diseases, US15722110, 2017.
Francone OL, Guey L, Holmes K, et al. Lipoprotein lipase for treatment of hypertriglyceridemic-related conditions including acute pancreatitis. US9597376B2, 2017.
Saxena RK. Microbial lipases: potential biocatalysts for the future industry. Curr Sci 1999; 77: 101-15.
Lott J, Lu C. Lipase isoforms and amylase isoen-zymes: assays and application in the diagnosis of acute pancreatitis. Clin Chem 1991; 37: 361-8.
Higaki S, Kitagawa T, Kagoura M, et al. Correlation between Propionibacterium acnes biotypes, lipase activity and rash degree in acne patients. J Dermatol 2000; 27: 519-22.
Simons JWF, Adams H, Cox RC, et al. The lipase from Staphylococcus aureus: expression in Escheri-chia coli, large-scale purification and comparison of substrate specificity to Staphylococcus hyicus lipase. Eur J Biochem 1996; 242: 760-9.
Chauhan M, Chauhan RS, Garlapati VK. Evaluation of a new lipase from Staphylococcus sp. for detergent additive capability. Biomed Res Int 2013 2013.
Jeon JH, Kim J-T, Kim YJ, et al. Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol 2009; 81: 865-74.
Weerasooriya M, Kumarasinghe A. Isolation of alkaline lipase from rubber seed-partial purification, characterization and its potential applications as a detergent additive. Indian J Chem Technol 2012; 19: 244-9.
Dey A, Chattopadhyay A, Saha P, et al. An Approach to the identification and characterisation of a psychrotrophic lipase producing Pseudomonas sp ADT3 from Arctic region. Adv Biosci Biotechnol 2014; 5: 322.
Aboualizadeh F, Kaur J, Behzad-Behbahani A, et al. Induction of mutation in Bacillus subtilis lipase gene using error-prone PCR. Jundishapur J Microbiol 2011; 4: 153-8.
Niyonzima FN, More SS. Coproduction of detergent compatible bacterial enzymes and stain removal evaluation. Basic Microbiol 2015; 55: 1149-58.
Su J, Zhang F, Sun W, et al. A new alkaline lipase obtained from the metagenome of marine sponge Ircinia sp. World J Microbiol Biotechnol 2015; 31: 1093-102.
Phuah E-T, Tang T-K, Lee Y-Y, et al. Review on the current state of diacylglycerol production using enzymatic approach. Food Bioprocess Technol 2015; 8: 1169-86.
Holland B, Bernhardt R, Sajic B. Cold-water laundry detergents. US0170369816, 2017.
Latha K. Studies on lipid acyl hydrolases during tea processing. Ann Plant Physiol 1999; 13: 73-8.
Webb C, Black GM, Atkinson B. Process engineering aspects of immobilised cell systems. 1st ed. Rugby, Warwickshire: Pergamon 1986.
Pilkington PH, Margaritis A, Mensour NA, et al. Fundamentals of immobilised yeast cells for continuous beer fermentation: a review. J Inst Brew 1998; 104: 19-31.
Mattiasson B. Immobilized cells and organelles. CRC Press 1983.

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Year: 2019
Page: [45 - 56]
Pages: 12
DOI: 10.2174/1872208312666181029093333
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