Login Register Cart 0
Generic placeholder image

Current Enzyme Inhibition

Editor-in-Chief

ISSN (Print): 1573-4080
ISSN (Online): 1875-6662

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Antispermatogenic Effect of Piper betel Leaf Stalk Extract with Reference to Kinetic Studies of 17β-hydroxy Steroid Dehydrogenase Enzyme in Testes of Albino Rats

Author(s): Govardhan Naik Amgoth, P. Venkataramanaiah and Changamma Challa*

Volume 15, Issue 1, 2019

Page: [36 - 40] Pages: 5

DOI: 10.2174/1573408014666181018113911

Price: $65

Abstract

Background: In the development and maintenance of male reproductive function and fertility, steroidogenesis plays a key role.

Aims: The aim of the present study was to investigate the effect of the betel leaf stalk extract on 17β- hydroxy steroid dehydrogenase activity levels.

Methods: The observed elevation in testicular cholesterol levels may be due to decreased androgen production, which resulted in impaired spermatogenesis. The decreased steroidogenic enzyme 17β- HSD activity represents decreased androgen production by the extract administration. Reduction in enzyme active site density and Km value revealed that there was a reduction in enzyme-substrate affinities and rate of E-S complex breakdown in the administered rat testes.

Results & Conclusion: The administration of betel leaf stalk extract resulted in decreased enzyme content probably through impaired synthesis.

Keywords: 17β-HSD, Betel leaf stalk extract, cholesterol, kinetic studies, spermatogenesis, steroidogenesis.

« Previous Next »
Graphical Abstract

[1]
Tewari, P.V.; Chaturvedi, C.; Dixit, S.N. Antifertility effect of betel leaf stalk (Tambul patrabrint): a preliminary experimental study. J. Res. Indian Med., 1970, 4(2), 143-151.
[2]
Sarkar, A.; Ramesh, R.; Somayajulu, B.L.K. Agnihotri R and Jull A J T. High resolution Holocene monsoon record from the eastern Arabian Sea. Earth Planet. Sci. Lett., 2000, 177, 209-218.
[3]
Ogbuewu, I.P.; Omede, A.A.; Uchegbu, M.C.; Okoli, I.C.; Iloeje, M.U. Clinical significance of dietary soy- isoflavones: A review.Proceedings of the International Conference on Global Food Crisis; Federal University of Technology Owerri: , 2009, 19, pp. (24)113-117.
[4]
Mathur, N.; Jain, G.C.; Pandey, G. Effect of Tecoma Stan leaves on the reproductive system of male albino rats. Int. J. Pharmacol., 2010, 6, 152-156.
[5]
Ogbuewu, I.P.; Ogundu, U.E.; Opara, M.N.; Okoli, I.C.; Umesiobi, D.O.; Herbert, U. Iloeje, M.U. Spermatozoa manipulation techniques: A current reproductive technology tool kit in reproductive physiology. A review. J. Med. Sci, 2010, 10(5), 110-123.
[6]
Ogbuewu, I.P.; Odoemenam, V.U.; Obikaonu, H.O.; Opara, M.N.; Emenalom, O.O.; Uchegbu, M.C.; Okoli, I.C.; Esonu, B.O.; Iloeje, M.U. The growing importance of neem (Azadirachta indica A. Juss) in agriculture, industry, medicine and environment: A review. Research. J. Med. Plant, 2011, 5(3), 230-245.
[7]
Verma, P.K.; Mathur, A.; Sharma, P.; Gupta, R.S.; Joshi, S.C.; Dixit, V.P. Effect of Sarcostemma acidumstem extract on spermatogenesis in male albino rats. Asian J. Androl., 2002, 4(1), 43-47.
[8]
Jie, H.U.; Zhang, Z.; Azhar, S. Cellular cholesterol delivery, intracellular processing and utilization for biosynthesis of steroid hormones. Nutr. Metab. (London)., 2010, 7, 47.
[9]
Emeruwa, A.C. Antibacterial substance from Carica papaya fruit extract. J. Nat. Products., 1982, 45, 123-127.
[10]
Dehghan, M.H.; Thomas Martin, M.A.; Dehghanan, R. Antifertility effect of neem seed alcoholic extract on epididymal sperm of mice. Iranian J. Reproductive Med., 2005, 3(2), 83-89.
[11]
Natelson. S. Total cholesterol procedure Liebermann-Burchard reagent. In: Techniques of clinical chemistry; Charles C. Thomas publishers, Springfield, Illinois, USA, 1971; 3, p. 263.
[12]
Bergmeyer, H.U. Methods of enzymatic analysis, HU Bergmeyer (ed) Verlag chemie Weinheim, Academic press, Inc., New York, Biol. Chem Hoppe Seyler 1974, 369(1), 733-40.
[13]
WHO: Protocol CG-40. Preparation of Alcoholic Extract for Bioassay and Photochemical Studies; World Health Organization, 1983.
[14]
Yakubu, M.T.; Akanji, M.A.; Oladiji, A.T. Effect of oral administration of aqueous extract of Fadogia agrestisstem on some testicular function indices of male rats. J. Ethno. Pharmacol., 2008, 111, 288-292.
[15]
Bedwall, R.S.; Edwards, M.S.; Katoch, M.; Bahuguna, A.; Dewan, R. Histological and biochemical changes in testes of zinc deficient BALB/C strain of mice. Indian J. Experimental. Biol., 1994, 32, 243-247.
[16]
Priyanka, R.; Priyanka, M.; Ruchisha, S. Effect of continuous intake of microwave exposed mice feed on the testicular glycogen and seminal vesicular fructose of swiss albino mice. World J. Pharmacy Pharmaceut. Sci., 2013, 2(5), 3728-3734.
[17]
Anitha, P.; Indira, M. Impact of feeding ethanolic extract of root bark of Cananga odoranta (LAM) on reproductive functions in male rats. Indian J. Exp. Biol., 2006, 44, 976-980.
[18]
Yakubu, M.T.; Akanji, M.A.; Oladiji, A.T. Evaluation of antiandrogenic potential of aqueous extract of Chromolaena odoratum (L) leaves in male rats. Andrologia, 2007, 39, 235-243.
[19]
Hammami, I.; Nahdi, A.; Mauduit, C.; Benahmed, M.; Amri, M.; Ben Amar, A.; Zekri, S.E.; May, A.E.; May, M.V. The inhibitory effects on adult male reproductive functions of crude garlic (Allium sativum) feeding. Asian J. Androl., 2008, 10, 593-601.
[20]
Sarkar, M.; Gangopadhyay, P.; Basak, B. The reversible antifertility effect of Piper betel Linn. On swiss albino male mice. Contraception, 2000, 62(5), 271-274.
[21]
Reddy, K.Y.V.; Reddy, S.P.; Shivalingam, M.R. Testosterone mediated partial recovery of carboplatin induced reproductive toxicity in male wistar rats. J. Biomed. Sci. Res, 2010, 2(1), 46-53.
[22]
Dupont, V.; Labrie, F.; Lvuthe, V.; Pelletier, G. Ontogeny of 3β- hydroxy steroid dehydrogenase/!5-!4isomerase (3β-HSD) in rat testis as studies by immunocyto chemistry. Anat. Embryol., 1993, 187, 583-589.
[23]
Lakshman, J., and ; Changamma, C. Antispermatogenic effect of carica papaya seed extract on steroidogenesis in albino rats. Int. J. Pharm. Pharm. Sci., 2013, 5(1), 67-69.
[24]
Akingbemi, B.T.; Braden, T.D.; Kemppainen, B.W.; Hancock, K.D.; Sherrill, J.D. Exposure to phytoestrogens in the perinatal period affects androgen secretion by testicular Leydig cells in the adult rat. Endocrinology, 2007, 148, 4475-4488.
[25]
Hancock, K.D.; Coleman, E.S.; Tao, Y.X.; Morrison, E.E.; Braden, T.D. Genistein decreases androgen biosynthesis in rat Leydig cells by interference with luteinizing hormone dependent signaling. Toxicol. Lett., 2009, 184, 169-175.

Rights & Permissions Print Cite
Article Metrics
70
3
1
1
© 2024 Bentham Science Publishers | Privacy Policy