Deregulation of miR-21 and miR-29a in Cervical Cancer Related to HPV Infection

Author(s): Sara Zamani, Amir Sohrabi*, Seyed Masoud Hosseini, Marjan Rahnamaye-Farzami, Abolfazl Akbari

Journal Name: MicroRNA

Volume 8 , Issue 2 , 2019

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Graphical Abstract:


Background: Early diagnosis is an important factor to improve the survival of Invasive Cervical Cancer (ICC) patients. Molecular biomarkers such as micro RNA (miRNA) can be used in the early detection of ICC. The expression of miR-21 and miR-29a are deregulated in many types of human cancers.

Objective: The aim of this study was to investigate the differences in miR-21 and miR-29a expression patterns in the Human Papilloma Virus (HPV) infection and various grades of cervical cancer among Iranian women.

Methods: Small RNAs were extracted from positive for HPV, cervical cancer and healthy samples from 43, 50 and 46 individuals, respectively. Expression levels of miR-21 and miR-29a were analyzed by SYBR Green real-time RT-PCR using specific primers, and 5s rRNA as the internal reference gene.

Results: Results have shown a significant increase in miR-21 and decrease in miR-29 in cancerous samples in comparison with the control groups (P < 0.0001).

Conclusion: This study illustrated that miR-21 and miR-29a could be operated as an oncogene and tumor-suppressor in cervical cancer progression. More studies are needed to demonstrate the role of miR-21 and miR-29a as potential biomarkers for the diagnosis of cervical cancer in future investigations.

Keywords: Biomarker, cervical cancer, HPV, infection, microRNA, miR-21, miR-29a.

Pedroza-Torres A, Lopez-Urrutia E, Garcia-Castillo V, et al. MicroRNAs in cervical cancer: evidences for a miRNA profile deregulated by HPV and its impact on radio-resistance. Molecules 2014; 19(5): 6263-81.
Jimenez-Wences H, Peralta-Zaragoza O, Fernandez-Tilapa G. Human papilloma virus, DNA methylation and microRNA expression in cervical cancer.[Review]. Oncol Rep 2014; 31(6): 2467-76.
Sohrabi A, Hajia M, Jamali F, Kharazi F. Is incidence of multiple HPV genotypes rising in genital infections? J Infect Public Health 2017; 10(6): 730-3.
Sohrabi A, Mirab-Samiee S, Modarressi MH, Izadimood N, Azadmanesh K, Rahnamaye-Farzami M. Development of in-house multiplex real time PCR for human papillomavirus genotyping in Iranian women with cervical cancer and cervical intraepithelial neoplasia. Asian Pac J Cancer Prev 2014; 15(15): 6257-61.
Wang N, Xu Z, Wang K, Zhu M, Li Y. Construction and analysis of regulatory genetic networks in cervical cancer based on involved microRNAs, target genes, transcription factors and host genes. Oncol Lett 2014; 7(4): 1279-83.
Hosseini N, Panahi M, Azadmanesh K, et al. Detection of mucosal type human papillomavirus in cutaneous squamous cell carcinoma in Iran. Pathol Res Pract 2013; 209(2): 90-4.
Sohrabi A, Hajia M. Cervical cancer and genital infections: assessment of performance and validation in human papillomavirus genotyping assays in Iran, its neighboring countries and Persian Gulf area. Iran J Pathol 2017; 12(1): 35-44.
Sohrabi A, Rahnamaye-Farzami M, Mirab-Samiee S, Mahdavi S, Babaei M. Comparison of in-house multiplex real time PCR, diagcor genoflow HPV array test and INNO-LiPA HPV genotyping extra assays with LCD-array kit for human papillomavirus genotyping in cervical liquid based cytology specimens and genital lesions in Tehran, Iran. Clin Lab 2016; 62(4): 615-9.
Sohrabi A, Mirab-Samiee S, Rahnamaye-Farzami M, et al. C13orf18 and C1orf166 (MULAN) DNA genes methylation are not associated with cervical cancer and precancerous lesions of human papillomavirus genotypes in Iranian women. Asian Pac J Cancer Prev 2014; 15(16): 6745-8.
Gomez-Gomez Y, Organista-Nava J, Gariglio P. Deregulation of the miRNAs expression in cervical cancer: human papillomavirus implications. BioMed Res Int 2013; 2013: 407052.
Zhu XL, Wen SY, Ai ZH, Wang J, Xu YL, Teng YC. Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer. Mol Med Rep 2015; 12(1): 55-62.
Granados-Lopez AJ, Lopez JA. Multistep model of cervical cancer: participation of miRNAs and coding genes. Int J Mol Sci 2014; 15(9): 15700-33.
Sohrabi A, Rahnamaye FM, Mirab SS, Modarresi MH. An overview on papillomaviruses as the main cause of cervical cancer. The Iranian J Obst. Gyne Infertility 2015; 18(145): 14-25.
Krichevsky AM, Gabriely G. miR-21: a small multi-faceted RNA. J Cell Mol Med 2009; 13(1): 39-53.
Liu J, Sun H, Wang X, et al. Increased exosomal microRNA-21 and microRNA-146a levels in the cervicovaginal lavage specimens of patients with cervical cancer. Int J Mol Sci 2014; 15(1): 758-73.
Servin-Gonzalez LS, Granados-Lopez AJ, Lopez JA. Families of microRNAs expressed in clusters regulate cell signaling in cervical cancer. Int J Mol Sci 2015; 16(6): 12773-90.
Yamamoto N, Kinoshita T, Nohata N, et al. Tumor-suppressive microRNA-29a inhibits cancer cell migration and invasion via targeting HSP47 in cervical squamous cell carcinoma. Int J Oncol 2013; 43(6): 1855-63.
Han Y, Xu GX, Lu H, et al. Dysregulation of miRNA-21 and their potential as biomarkers for the diagnosis of cervical cancer. Int J Clin Exp Pathol 2015; 8(6): 7131-9.
Bumrungthai S, Ekalaksananan T, Evans MF, et al. Up-regulation of miR-21 is associated with cervicitis and human papillomavirus infection in cervical tissues. PLoS One 2015; 10(5): e0127109.

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Article Details

Year: 2019
Published on: 26 February, 2019
Page: [110 - 115]
Pages: 6
DOI: 10.2174/2211536607666181017124349

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