Background: Grindstone technique has been widely used as an efficient, consistent,
more environmentally benign, solvent-free protocol for the preparation of many compounds with
higher atom economy.
Methods: A series of fourteen piperonal chalcone compounds were synthesized by this method and
characterized by physical and spectral data (FT-IR, 1H NMR, Mass and elemental analysis). All
chalcones were evaluated for their cytotoxic action against the cancer cell lines, MCF-7 and HepG2.
One 2-pyridyl-substituted compound 14 with IC50 values 17.4±0.2 towards MCF-7 and
15.4±0.6µmol L-1 towards HepG2 cells.
Results: The results demonstrated that the cytotoxic activity of 2-pyridyl-substituted compound
shown higher activity as compared with the standard cisplatin towards HepG2 cells.
Conclusion: Compound 14 showed good antioxidant activities in the DPPH test and H2O2 assay
(IC50 = 17.23± 33/µg/mL and 20.17± 0.33µg/mL) when compared with the standard ascorbic acid
(IC50=µg/mL 18.26 ± 0.22and 21.66± 1.06 µg/mL).