Background: MicroRNA-19b (miR-19b) is essential in determining
oligodendroglia proliferation. Phosphatase and tensin homologue on chromosome 10
(PTEN) is considered the target of miR-19b and participates in oligodendrocyte
differentiation and proliferation.
Methods: Murine EAE was induced by myelin oligodendrocyte glycoprotein (MOG35–
55). For EAE reversal, artificially synthesized agomiR-19b was intravenous injected after
Results: We found that the expression of miR-19b is significantly reduced in an
experimental autoimmune encephalomyelitis (EAE) mouse model. This downregulation,
which is associated with the neurological scores, can be dramatically ameliorated by
agomiR-19b. Our results show that agomiR-19b increases the expression of myelin
basic protein (MBP) and cyclic nucleotide phosphodiesterase (CNP), which are regularly
utilized as molecular markers of oligodendrocytes. Furthermore, our study also revealed
that miR-19b probably affects the expression of PTEN in the EAE model.
Conclusion: These results indicate that the restoration of miR-19b probably exerts its
therapeutic effect by affecting PTEN in the pathogenesis of EAE.