The Thermodynamic and pH metric Studies on the Binding of Hg+2 and Mo+2 with RNA by Polarographic and Spectrometric Techniques

Author(s): Shveta Acharya, Arun Kumar Sharma*

Journal Name: Current Physical Chemistry

Volume 8 , Issue 3 , 2018

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Graphical Abstract:


Background: Metalloproteins and metal-protein complexes play key roles in all organisms. For example, certain metalloproteins are involved in metal homeostasis and detoxification, or oxidative stress protection; whereas, metals serve as essential cofactors in a large number of metal-protein complexes.

Objective: Present work attempts to critically review methods for assessing the structure, characterization of the metal binding sites, with RNA involved in the various process of a living being are discussed.

Method: The binding of Hg(II) and Mo (II) ions have been studied with RNA at different pH values and temperatures by the polarographic and spectrometric techniques. It is observed that binding data depend on pH and temperature.

Results: The intrinsic association constants (k) and the number of binding sites (n) were calculated from Scatchard plots. The free energies of aggregation, ΔG associated with the binding interaction of the Hg (II) and Mo(II) and RNA were calculated. The negative values of the ΔG confirm the feasibility of interaction between the metals and RNA.

Conclusion: All the observations recorded in this paper indicate that the Hg (II) and Mo (II) ions have a good affinity of binding with RNA and the number of binding sites is dependent on various physical and chemical factors. Both ions when bind with the RNA induce metabolic function at tissue level in fishes in the aquatic environment.

Keywords: Gibbs free energy, Mercury (II), Molybdate (II), pH, polarographic, RNA, scatchard plots.

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Article Details

Year: 2018
Published on: 18 January, 2019
Page: [186 - 193]
Pages: 8
DOI: 10.2174/1877946808666181002102441
Price: $25

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