Title:Identification of Candidate Biomarkers for HCV Leading to Hepatocellular Carcinoma Differential Stages From Serum Samples
VOLUME: 16 ISSUE: 3
Author(s):Amber Afroz*, Saba Saleem, Kalsoom Sughra, Sabaz Ali Khan and Nadia Zeeshan*
Affiliation:Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Gujrat, Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Gujrat, Biotechnology Program, Department of Environmental Sciences COMSATS Institute of Information Technology, Abbottabad, Department of Biochemistry and Biotechnology, University of Gujrat, Hafiz Hayat Campus Gujrat, Gujrat
Keywords:Hepatocellular carcinoma, vitronectin, glypican 3, clusterin, SDS-PAGE, glutathione S transferase.
Abstract:
Background: Hepatocellular carcinoma (HCC) is one of the most deadly liver malignancy
found and Hepatitis C virus (HCV) is a prominent risk factor for this disease. Prognosis of HCC is poor;
initiate the need of markers to discover therapeutic targets in HCC.
Introduction: Clinical staging systems of HCC composed of tumor characteristics along with liver
function test are important in prognosis but they are not precise. Molecular profiling can lead to a better
understanding of the physiopathology of HCC and can help in the development of novel therapeutic
approaches.
Methods: 64 HCC serum samples (shifted from HCV) were graded into stage I- IV; along with +ive (3
Hepatitis C) and -ive control (2 healthy persons). Proteins were separated by sodium dodecyl sulfatepolyacrylamide
gel electrophoresis (SDS-PAGE) and differential mRNA expression from serum samples
of different HCC stages was confirmed by Real Time Polymerase Chain Reaction (qPCR).
Results: HCC serum proteins displayed differential expression of glutathione s-transferase (GST),
glypican-3 (GPC3), vitronectin (VTN), and clusterin (CLU) by SDS-PAGE. GST was expressed in -ive
control, while GPC3 was found in both -ive and +ive control. The qPCR analysis, display more than
0.07 fold decrease in GST in I-IV HCC stages. The highest increase in HCC stages was observed by
GPC3; about 4 fold increase in I-IV stages. VTN show 1.7-3.4 fold; while CLU show 2-3.5 fold increase
in four stages of HCC.
Conclusion: GPC3, VTN and CLU in combination can be good potential markers for differentiating
stages (I-IV) of HCC.
