Title:Modified LDL Particles Activate Inflammatory Pathways in Monocyte-derived Macrophages: Transcriptome Analysis
VOLUME: 24 ISSUE: 26
Author(s):Alexander N. Orekhov*, Yumiko Oishi , Nikita G. Nikiforov, Andrey V. Zhelankin, Larisa Dubrovsky, Igor A. Sobenin, Alexander Kel, Daria Stelmashenko, Vsevolod J. Makeev, Kathy Foxx, Xueting Jin, Howard S. Kruth and Michael Bukrinsky
Affiliation:Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 125315 Moscow, Department of Cellular and Molecular Medicine, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 1138510, Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 125315 Moscow, Laboratory of postgenomic research, Federal Research and Clinical Center of Physical-Chemical Medicine, 119435 Moscow, GW School of Medicine and Health Sciences, George Washington University, Washington, DC 20037, Laboratory of Medical Genetics, Institute of Experimental Cardiology, National Medical Research Center of Cardiology, 121552 Moscow, Biosoft.ru Ltd, 630001 Novosibirsk, Biosoft.ru Ltd, 630001 Novosibirsk, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow 119991, Kalen Biomedical, LLC, Montgomery Village, MD 20886, Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, Experimental Atherosclerosis Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, GW School of Medicine and Health Sciences, George Washington University, Washington, DC 20037
Keywords:Atherosclerosis, inflammation, macrophages, LDL, modified LDL, pro-inflammatory signaling.
Abstract:Background: A hallmark of atherosclerosis is its complex pathogenesis, which is dependent on altered
cholesterol metabolism and inflammation. Both arms of pathogenesis involve myeloid cells. Monocytes migrating
into the arterial walls interact with modified low-density lipoprotein (LDL) particles, accumulate cholesterol and
convert into foam cells, which promote plaque formation and also contribute to inflammation by producing proinflammatory
cytokines. A number of studies characterized transcriptomics of macrophages following interaction
with modified LDL, and revealed alteration of the expression of genes responsible for inflammatory response and
cholesterol metabolism. However, it is still unclear how these two processes are related to each other to contribute
to atherosclerotic lesion formation.
Methods: We attempted to identify the main mater regulator genes in macrophages treated with atherogenic
modified LDL using a bioinformatics approach.
Results: We found that most of the identified genes were involved in inflammation, and none of them was
implicated in cholesterol metabolism. Among the key identified genes were interleukin (IL)-7, IL-7 receptor, IL-
15 and CXCL8.
Conclusion: Our results indicate that activation of the inflammatory pathway is the primary response of the
immune cells to modified LDL, while the lipid metabolism genes may be a secondary response triggered by
inflammatory signalling.
