Background: Non-coding small RNAs are involved in organism development,
and their aberrant regulation induces various diseases, including hepatocellular
carcinoma (HCC), but their exact mechanisms have not been determined.
Objective: The aim was to investigate the role of miR-142-3p on HMGB1 expression in
Methods: Expression levels of miR-142-3p in HCC tissues and cultured cells were
measured by RT-PCR. The invasion and metastasis abilities of HepG2 cells according
to Transwell migration and invasion assays, and protein expression was measured by
Results: The present study reported that miR-142-3p promotes the invasion and
migration of HCC cells. miR-142-3p levels are lower in HCC tissues than in adjacent
non-cancerous tissues, suggesting a tumor suppressor role for miR-142-3p. Highmobility
group box protein 1 (HMGB1) is an oncogene that promotes the metastasis of
HCC. miR-142-3p or HMGB1 knockdown alone inhibits the invasion and migration of
HCC cells, and HMGB1 overexpression impedes the effect of miR-142-3p. Further
studies showed that HMGB1 is a direct target gene of miR-142-3p in HCC. miR-142-3p
represses HMGB1 gene transcription by directly binding to the 3′ untranslated region
(UTR) of HMGB1, thereby inhibiting cancer cell invasion and migration.
Conclusion: This study, for the first time, reports that miR-142-3p is a novel tumor
suppressor that inhibits the invasion and migration of HCC cells by directly regulating
gene transcription of HMGB1. Thus, miR-142-3p may be a potential diagnostic and
therapeutic biomarker for HCC patients.