Marine Streptomyces sp. VITASP as a Source of New Bioactive Secondary Metabolites

Author(s): Aditi Thosar, Pooja Satpathy, Jemimah Naine S., Chandrasekaran S. Devi*

Journal Name: Current Bioactive Compounds

Volume 16 , Issue 5 , 2020


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Graphical Abstract:


Abstract:

Background: The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces sp. VITASP with a view to assess their therapeutic potential.

Methods: The morphological, physiological and the biochemical properties of the strain Streptomyces sp. VITASP were confirmed by conventional methods. The present study evaluated the antibacterial, antioxidant and cytotoxic activities.

Results: The isolate was identified to be Streptomyces sp. (Genbank accession number: KR233807). The ethyl acetate extract of Streptomyces sp. VITASP showed maximum antibacterial activity of two Gram- positive and Gram- negative bacteria at 0.5mg/mL. The antioxidant potential of the crude extract exhibited strong reducing power activity at 0.5 mg/mL with 93±0.05% inhibition. The antiinflammatory and anti-diabetic properties were identified at 0.5 mg/mL concentration. The cytotoxic effect was found with an IC50 of 500μg/ mL on HeLa cell lines. The GC-MS analysis and the chromatogram patterns revealed major peak at 18.485 which corresponds to Pyrrolo[1,2-a]pyrazine-1,4-dione, Hexahydro-3-(2-methylpropyl. IR spectra showed the functional groups. HPLC Retention time of the peak was found to be 2.414 min.

Conclusion: This work demonstrates that the extract should be considered as a useful source as an antioxidant and antimicrobial agent and gives further insight into the potential use of the compounds as drugs for various other bioactivities.

Keywords: Marine Streptomyces, metabolites, bioactivity, antimicrobial drug discovery, anti-cancer agents, actinomycetes.

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Article Details

VOLUME: 16
ISSUE: 5
Year: 2020
Published on: 16 July, 2020
Page: [611 - 617]
Pages: 7
DOI: 10.2174/1573407214666180904100049
Price: $65

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