Background: Various analogues of benzimidazole are found to be biologically and therapeutically
potent against several ailments. Benzimidazole when attached with heterocyclic rings has
shown wide range of potential activities. So, from the above provided facts, we altered benzimidazole
derivatives so that more potent antagonists could be developed. In the search for a new category of antimicrobial
and anticancer agents, novel azomethine of 2-mercaptobenzimidazole derived from 3-(2-
(1H-benzo[d]imidazol-2-ylthio)acetamido)benzohydrazide were synthesized.
Results and Discussion: The synthesized analogues were characterized by FT-IR, 1H/13C-NMR and
MS studies as well C, H, N analysis. All synthesized compounds were evaluated for in vitro antibacterial
activity against Gram-positive (B. subtilis), Gram-negative (E. coli, P. aeruginosa, K. pneumoniae and S.
typhi) strains and in vitro antifungal activity against C. albicans and A. niger strains by serial dilution
method, the minimum inhibitory concentration (MIC) described in μM/ml. The in vitro anticancer activity
of synthesized compounds was determined against human colorectal carcinoma cell line (HCT-
116) using 5-fluorouracil as standard drug.
Conclusion: In general, most of the synthesized derivatives exhibited significant antimicrobial and anticancer
activities. Compounds 8, 10, 15, 16, 17, 20 and 22 showed significant antimicrobial activity
towards tested bacterial and fungal strains and compound 26 exhibited significant anticancer activity.