Chirality is now a key area in the field of research. Researchers are well versed with analytical
separations but at the same time, they are unaware of the growth in the field of chiral separation.
According to the United States Food and Drug Administration (US FDA) guidelines, it is mandatory to
separate chiral drugs before they are marketed. Chiral separation has gained importance in the last 10
years due to the differential biological responses of the enantiomers in chiral environment. Identical
physical and chemical properties of the enantiomers pose a major challenge for the separation of chiral
compounds. Further, bioanalytical method development is also necessary to throw light on the fate of
separated enantiomers in biological environment. High Performance Liquid Chromatography (HPLC)
and Capillary Electrophoresis (CE) are the most widely used techniques for such separation. Antihistamines
are a class of drugs that are represented by a wide number of chiral compounds. Hence this review
focuses on enantioseparation of chiral antihistamine drugs. It begins with a brief discussion about
antihistamine drugs, chiral separation and its need for study, followed by a brief overview of the analytical
and bio-analytical work carried out on different chiral antihistamine drugs. The analytical and
bio-analytical techniques that are used include HPLC, CE and some new techniques.
Keywords: Antihistamine drugs, chiral, Capillary Electrophoresis (CE), High-Performance Liquid Chromatography (HPLC),
Subcritical Fluid Chromatography (SFC), Gas Chromatography (GC).
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