The Effect of Zinc Oxide Nanoparticles (ZnO NPs) on Vigna mungo L. Seedling Growth and Antioxidant Activity

Author(s): Kantabathini Venkata Pavani*, Mallula Beulah, Govinda Udayar Sai Poojitha.

Journal Name: Nanoscience & Nanotechnology-Asia

Volume 10 , Issue 2 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Aim: The purpose of this study was to test the phytotoxicity effect of ZnONPs on Vigna mungo L. seedling growth and antioxidant activity.

Methods: Vigna mungo L. Seeds were treated with to a wide range of ZnO NPs ranging 5 to25mg/100ml for 8hours. Vigna mungo seeds that were soaked in ZnO NPs solution were sown in pots (20 cm × 40 cm) filled with red soil and a layer of coco peat. The effect of ZnO NPs on morphological, biochemical and antioxidant activity in Vigna mungo L. plants was investigated after 15,30,45 and 60 days.

Results: The impact of ZnO NPs on plant growth characteristics and biochemical changes in Vigna mungo L. plants was investigated after 15,30,45 and 60 days. The ZnONPs exposure significantly enhanced germination percentage by 111.3% but root length (75.25%), shoot length (89.81%), number of leaves (91.66%), length of leaves (76%), width of leaves (67.27%), fresh weight of plant (27.96%) and dry weight of plant (28.23%) decreased in the treated plants after 60 days exposure to 25mg/100ml compared to the untreated control. Interestingly, treated plants after 60 days exposure to 25mg/100ml increased significantly the chlorophyll (115.0%), reducing sugars (244.4%), total sugars (212.72%) protein (181.8%). Treatment to Vigna mungo L. seeds with ZnONPs has been found to induce the activities of antioxidant enzymes such as Guaiacol peroxidase, Glutathione Reductase, Catalase and increase in the ascorbic acid and hydrogen peroxide contents. TEM images revealed that the aggregated ZnO NPs to be deposited inside the seed.

Conclusion: Vigna mungo seeds treated with different concentrations of ZnO NPs showed decreased root growth and increased germination index, shoot and leaf growth. There was a significant change in Glutathione reductase, Guaiacol peroxidase and Catalase activity and ascorbic acid and hydrogen peroxide of Vigna mungo exposed to ZnONPs. Aggregated nanoparticles penetration into the intracellular region of the seed was observed.A complete study on the toxic effects of ZnO NPs can help significantly in the safe disposal of ENPs for the reduction of adverse effects in both environmental and agricultural systems.

Keywords: ZnO NPs, Vigna mungo L., seedling growth, antioxidant activity, TEM, nanotechnology.

[1]
Nair, R.; Varghese, S.H.; Nair, B.G.; Maekawa, T.; Yoshida, Y.; Kumar, D.S. Nano particulate material delivery to plants. Plant Sci., 2010, 179, 154-163.
[2]
Soppimath, K.S.; Aminabhavi, T.M.; Kulkarni, A.R.; Rudzinski, W.E. Biodegradable polymeric nanoparticles as drug delivery devices. J. Control. Release, 2001, 70, 1-20.
[3]
Wiesner, M.R.; Lowry, G.V.; Alvarez, P.; Dionysiou, D.; Biswas, P. Assessing the risks of manufactured nanomaterials. Environ. Sci. Technol., 2006, 40, 4336-4345.
[4]
Galbraith, D.W. Nanobiotechnology: Silica breaks through in plants. Nat. Nanotechnol., 2007, 2, 272-273.
[5]
Torney, F.; Trewyn, B.G.; Lin, V.S.Y.; Wang, K. Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat. Nanotechnol., 2007, 2, 295-300.
[6]
Ma, X.; Geiser-Lee, J.; Deng, Y.; Kolmakov, A. Interactions between engineered nanoparticles (ENPs) and plants: Phytotoxicity, uptake and accumulation. Sci. Total Environ., 2010, 408, 3053-3061.
[7]
Prasad, T.N.V.K.V.; Sudhakar, P.; Sreenivasulu, Y.; Latha, P.; Munaswamy, V.; Reddy, K.R.; Sreeprasad, T.S.P.; Sajanlal, R.; Pradeep, T. Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. J. Plant Nutr., 2012, 35, 905-927.
[8]
Sedghi, M.; Hadi, M.; Toluie, S.G. Effect of nano zinc oxide on the germination of soybean seeds under drought stress. Ann. West Uni. Timis Oara Ser Biol. XVI, 2013, 2, 73-78.
[9]
Ramesh, M.; Palanisamy, K.; Babu, K.; Sharma, N.K. Effects of bulk & nano-titanium dioxide and zinc oxide on physio-morphological changes in Triticum aestivum Linn. J. Glob. Biosci, 2014, 3, 415-422.
[10]
Raskar, S.V.; Laware, S.L. Effect of zinc oxide nanoparticles on cytology and seed germination in onion. Int. J. Curr. Microbiol. Appl. Sci., 2014, 3, 467-473.
[11]
Lowry, O.H.; Rosebrough, N.J.; Farr, N.J.; Randall, R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem., 1951, 193, 265-275.
[12]
AOAC Official methods of analysis. In: Official Methods of Analysis, 21st ed.; , 2019. Available from. https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/
[13]
Chance, B.; Maehly, A.C. Assay of catalase and peroxidase. Meth. Enzymol., 1955, 2, 764-775.
[14]
Aebi, H. Catalase in vitro. In: Methods in Enzymology, Oxygen Radicals in Biological systems; L., Packer., Ed.; Academic Press: Orlando, FL, 1984; pp. 121-126.
[15]
Carlberg, I.; Mannervik, B. Glutathione reductase. In: Methods in Enzymology; Academic Press: New York, USA, 1985; pp. 484-490.
[16]
Velikova, V.; Yordanov, I.; Edreva, A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: Protective role of exogenous poly-amines. Plant Sci., 2000, 151, 59-66.
[17]
Oser, B.L. Hawk's physiological chemistry; Mc Graw Hill; New Yor: USA, 1979, pp. 702-705.
[18]
Spur, A. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res., 1969, 26, 311.
[19]
John, J.B.; Lonnie, D.R. In: Electron Microscopy Principles and Techniques for Biologists. 2nd edn.. Jones and Bartlett Publishers: Sudbury, Massachusetts, 1998; pp. 72-144.
[20]
Zheng, l.; Mingyu, S.; Xiao, W.; Chao, L.; Chunxiang, Q.; Liang, C.; Huang, H.; Xiaoqing, L.; Hong, F. Effect of nano-anatase on spectral characteristics and distribution of LHCLL on the thylakoid memberance of spinach. Biol. Trace Elem. Res., 2007, 120, 273-280.
[21]
Sresty, T.V.S.; Rao, K.V.M. Ultrastructural alterations in response to zinc and nickel stress in the root cells of pigeonpea. Environ. Exp. Bot., 1999, 41, 3-13.
[22]
Lin, D.; Xing, B. Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environ. Pollut., 2007, 150, 243-250.
[23]
Kumar, S.; Patra, A.K.; Datta, S.C.; Rosin, K.G.; Purakayastha, T.J. Phytotoxicity of nanoparticles to seed germination of plants. Int. J. Adv. Res., 2015, 3, 854-865.
[24]
Cakmak, I. Role of zinc in protecting plant cells from reactive oxygen species. New Phytol., 2000, 146, 185-205.
[25]
Sharma, P.; Bhatt, D.; Zaidi, M.G.; Saradhi, P.P.; Khanna, P.K.; Arora, S. Silver nanoparticlemediated enhancement in growth and antioxidant status of Brassica juncea. Appl. Biochem. Biotechnol., 2012, 167, 2225-2233.
[26]
Sasan, M.; Sara, S.M. Zinc sulphate and nano-zinc oxide effects on some physiological parameters of Rosmarinus officinalis. Astrophys. J. Suppl., 2017, 8, 2635-2649.
[27]
Tuteja, N. Mechanisms of high salinity tolerance in plants. Methods Enzymol., 2007, 428, 419-438.
[28]
Gunjan, B.; Zaidi, M.G.H.; Sandeep, A. Impact of gold nanoparticles on physiological and biochemical characteristics of Brassica juncea. J. Plant Biochem. Physiol., 2014, 2, 113.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 10
ISSUE: 2
Year: 2020
Page: [117 - 122]
Pages: 6
DOI: 10.2174/2210681208666180820150647
Price: $25

Article Metrics

PDF: 11
HTML: 3