Fabrication and Characterization of PANI/Ag Nanocomposites Voltammetric Sensor for Foodborne Bacteria

Author(s): V. Chaitra*, V. Uma, S. Raja

Journal Name: Nanoscience & Nanotechnology-Asia

Volume 10 , Issue 1 , 2020

Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Introduction: Foodborne diseases are caused by consuming contaminated foods and/or beverages.

Methods: Traditional detection methods for foodborne bacteria are sluggish and laborious. In this study, room temperature voltammetric sensors with low cost, specific, rapid and easy detection were fabricated using Polyaniline (PANI) and silver (Ag) nanoparticles. PANI films were coated on to the Indium Tin Oxide (ITO) glass substrate using electrochemical deposition technique. PANI surface was modified using Ag nanoparticles prepared by reduction method as Ag is one of the most powerful disinfectants against microbes. Both surface electron microscopy (SEM), X-ray diffraction (XRD) technique revealed the presence of Ag nanoparticles in the composites. The peaks observed in Fourier Transform Infrared Spectroscopy (FTIR) and optical absorption spectra are characteristics to PANI/Ag nanocomposites.

Results: The antibacterial activities of the PANI/Ag nanocomposites were evaluated against Escherichia coli (E. Coli) (NCIM 2065), Staphylococcus aureus (S. aureus) (NCIM 2079) and Bacillus cereus (B. cereus) (NCIM 2106) using disk diffusion method. The composites showed better antibacterial activity due to the presence of Ag in comparison to pure PANI films.

Conclusion: The sensor current for composites was found to increase with the presence of all the microbes. The sensitivity of the sensor was higher for E. coli bacteria among the other bacterial strains.

Keywords: Polyaniline, Ag nanoparticles, antibacterial activity, E. coli, S. aureus and B. cereus.

Shukla, S.K. Vamakshi.; Minakshi.; Bharadavaja A.; Shekhar A.; Tiwari, A. Fabrication of electro-chemical humidity sensor based on zinc oxide/polyaniline nanocomposites. Adv. Mater. Lett., 2012, 3(5), 421-425.
Umare, S.S.; Shambharkar, B.H.; Ningthoujam, R.S. Synthesis and characterization of polyaniline-Fe3O4 nanocomposite electrical conductivity, magnetic, electrochemical studies. Synth. Met., 2010, 160(17-18), 1815-1821.
Choudhury, A. Polyaniline/silver nanocomposites: Dielectric properties and ethanol vapour sensitivity. Sens. Actuators B Chem., 2009, 138, 318.
Sivaraman, P.; Kushwaha, R.K.; Shashidhara, K.; Hande, V.R.; Thakur, A.P.; Samui, A.B.; Khandpekar, M.M. All solid supercapacitor based on polyaniline and crosslinked sulfonated poly [ether ether ketone]. Electrochim. Acta, 2010, 55(7), 2451-2456.
Shumaila, G.B.; Lakshmi, V.S.; Alam, M.; Siddiqui, A.M.; Zulfequar, M.; Husain, M. Synthesis and characterization of se doped polyaniline. Curr. Appl. Phys., 2010, 11(2), 217-222.
Rai, M.; Yadav, A.; Gade, A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv., 2009, 27, 76-83.
Ćirić-Marjanović, G. Recent advances in polyaniline composites with metals, metalloids and nonmetals. Synth. Met., 2013, 170, 31-56.
Reda, S.M.; Al-Ghannam, S.M. Synthesis and electrical properties of polyaniline composite with silver nanoparticles. Adv. Mater. Phys. Chem, 2012, 2, 75.
Barkade, S.S.; Naik, J.B.; Sonawane, S.H. Ultrasound assisted miniemulsion synthesis of polyaniline/Ag nanocomposite and its application for ethanol vapor sensing. Colloids Surf. A Physicochem. Eng. Asp., 2011, 378, 94.
An, J.; Luo, Q.; Li, M.; Wang, D.; Li, X.; Yin, R. A facile synthesis of high antibacterial polymer nanocomposite containing uniformly dispersed silver nanoparticles. Colloid Polym. Sci., 2015, 293(7), 1997-2008.
Poyraz, S.; Cerkez, I.; Huang, T.S.; Liu, Z.; Kang, L.; Luo, J.; Zhang, X. One-step synthesis and characterization of polyaniline nanofiber/silver nanoparticle composite networks as antibacterial agents. ACS Appl. Mater. Int., 2014, 6(22), 20025-20034.
Guzman, M.G.; Dille, J.; Godet, S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. World Acad. Sci. Eng. Technol., 2008, 43, 357-364.
Chaitra, V.; Pradeep, N.; Grace, A.N.; Uma, V.; Sellappan, R. Electrodeposition and characterization of polyaniline films for the detection of Staphylococcus aureus bacteria in food products. Sens. Lett., 2017, 15, 65-70.
Boomi, P.; Prabu, H.G.; Mathiyarasu, J. Synthesis and characterization of polyaniline/Ag–Pt nanocomposite for improved antibacterial activity. Colloids Surf. B Biointerfaces, 2013, 103, 9-14.
Ghaffari-Moghaddam, M.; Eslahi, H. Synthesis, characterization and antibacterial properties of a novel nanocomposite based on polyaniline/polyvinyl alcohol/Ag. Arab. J. Chem., 2014, 7, 846-855.
Singh, R.P.; Tiwari, A.; Pandey, A.C. Silver/polyaniline nanocomposite for the electrocatalytic hydrazine oxidation. J. Inorg. Organomet. Polym. Mater., 2011, 21(4), 788-792.
Pouget, J.P.; Jozefowicz, M.E.; Epstein, A.J.; Tang, X. MacDiarmid, X-ray structure of polyaniline. Macromolecules, 2011, 24(3), 779-789.
Saini, I.; Rozra, J.; Chandak, N.; Aggarwal, S.; Sharma, P.K.; Sharma, A. Tailoring of electrical, optical and structural properties of PVA by addition of Ag nanoparticles. Mater. Chem. Phys., 2013, 139, 802-810.
Ali, Y.; Kumar, V.; Dhaliwal, A.S.; Sonkawade, R.G. Surface modification of polyaniline nanofiber using silver nanoparticles to enhance sensing properties. Adv. Mater. Lett., 2013, 4, 368-372.
Hussain, J.I.; Kumar, S.; Hashmi, A.A.; Khan, Z. Silver nanoparticles: preparation, characterization, and kinetics. Adv. Mater. Lett., 2011, 2(3), 188-194.
Abdulla, H.S.; Abbo, A.I. Optical and electrical properties of thin films of polyaniline and polypyrrole. Int. J. Electrochem. Sci., 2012, 7, 10666-10678.
Ruparelia, J.; Chatterjee, A.; Duttagupta, S.; Mukherji, S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater., 2008, 4, 707-716.
Kucekova, Z.; Kasparkova, V.; Humpolicek, P.; Sevcikova, P.; Stejskal, J. Antibacterial properties of polyaniline–silver films. Chem. Pap., 2013, 67(8), 1103-1108.
Raffi, M.; Hussain, F.; Bhatti, L.; Akhter, J.; Hameed, A.; Hasan, M. Antibacterial characterization of silver nanoparticles against E. Coli ATCC-15224. J. Mater. Sci. Technol., 2008, 24(2), 192-196.
Guo, L.; Yuan, W.; Lu, Z.; Li, C.M. Polymer/nanosilver composite coatings for antibacterial applications. Colloids Surf. A Physicochem. Eng. Asp., 2013, 439, 69-83.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2020
Published on: 23 January, 2020
Page: [51 - 56]
Pages: 6
DOI: 10.2174/2210681208666180813110343
Price: $25

Article Metrics

PDF: 16