Characterization of Recombinant Phascolosoma esculenta Ferritin as an Efficient Heavy Metal Scavenger

Author(s): Hongwei Ding, Jun Zhou, Yu Han, Xiurong Su*

Journal Name: Protein & Peptide Letters

Volume 25 , Issue 8 , 2018

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Graphical Abstract:


Background: With economic development, the amount of industrial wastewater discharged with heavy metal ions increases, and heavy metal ion pollution in the environment worsens. Currently, this environmental issue has attracted much attention triggering the development and implication of pollutant binding- or degradation-related genes, especially in heavy metal ion detoxification. Additionally, accumulative results have shown that natural ferritin could store multiple toxic metal ions due to its great storage capacity, resulting in heavy metal detoxification in vivo.

Objective: In this study, the function of recombinant Phascolosoma esculenta ferritin as an efficient heavy metal scavenger was investigated.

Method: In this study, a recombinant ferritin (rferritin) was purified. The structure and heavy metal chelating capacity were examined via SEM, EDS, AFM and ICP-MS. The protection of rferritin on heavy metal toxicity was identified via cell experiments.

Results: The rferritin was expressed by E. coli host. The surface morphology and nanoscale structure of rferritin and human ferritin were changed after different heavy metal treatment, and similar protein aggregates were observed under same heavy metal treatment. ICP-MS analysis revealed the enrichment capacity of rferritin varied among different heavy metals and the cell experiments further confirmed that rferritin could protect the bone marrow mesenchymal stem cells against heavy metal toxicity.

Conclusion: The rferritin showed an excellent ability to enrich different heavy metals. The adsorption process featured stability, a high yield, simple implementation and low-cost. Thus, the rferritin shows significant potential in the manufacturing of drugs for heavy metal detoxification and environmental remediation.

Keywords: Phascolosoma esculenta, ferritin, heavy metal, cell protection, environmental remediation, detoxification.

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Article Details

Year: 2018
Page: [767 - 775]
Pages: 9
DOI: 10.2174/0929866525666180806111756
Price: $65

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