Title:Optimization of Total Phenol and Tannin Content and Biological Activity of <i>Dittrichia graveolens</i> (L.) GREUTER.
VOLUME: 16 ISSUE: 2
Author(s):Mahsa Souri and Alireza Shakeri*
Affiliation:School of Chemistry, Collage of Science, University of Tehran, Tehran, School of Chemistry, Collage of Science, University of Tehran, Tehran
Keywords:Microwave assisted extraction, ultrasound assisted extraction, phenol, tannis, antioxidant, antibacterial.
Abstract:
Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean
regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total
Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for
Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite
Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in
different methods on TTC and TPC. The antioxidant activities and antibacterial activities were
evaluated.
Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted
extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities
were studied using DPPH reagent and disc diffusion method was used to study the antibacterial
activities.
Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and
11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction.
It also indicated that MAE was the most effective method in comparison to UAE and maceration.
The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract
(IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL).
Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher
total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant
activities. The antibacterial test showed the great potential of this plant as an antibacterial compound
resource against different bacteria.