A Holistic View to Develop Descriptive Sheets for Argentinean Clover and Eucalyptus Unifloral Honey

Author(s): María C. Ciappini*, Amalia Calviño

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 6 , 2020

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Graphical Abstract:


Background: Honey is a complex natural product that varies not only in sensory properties but also in chemical composition. The apiculture industry is interested in offering unifloral honey with specific palynological, physicochemical and sensory characteristics. However, to the best of our knowledge, no proper study has been reported in Argentina in this regard so far.

Objective: The aim of this study was to develop descriptive sheets analyzing and compiling previous palynological, physicochemical and sensory data obtained from Argentine eucalyptus and clover unifloral honeys.

Methods: The analysis included predominant and main secondary pollens; physicochemical characteristics as moisture content, acidity, ash content, electrical conductivity, fructose, glucose, maltose and sucrose, optical rotation and colour; antioxidant indicators as DPPH, TEAC, FRAC, OH; content of phenolic and flavonoids compounds and the main sensory descriptors like colour, smell, aromatic notes, sweetness and persistence.

Results: The analysis was made by gathering 30 Eucalyptus honey (Eucalyptus spp.) and 55 clover honey (Trifolium sp.). Forty-one morphological pollen types were recognized. The set of physicochemical measures for honey met quality specifications for international marketing. Colour and odour intensity, higher for eucalyptus honey, were identified as the main differentiating sensory attributes.

Conclusion: An holistic analysis of all these properties allows to determine quality and certification of Argentinean eucalyptus and clover unifloral honey. The intervals of variation of selected palynological, physicochemical and sensory variables gathered in the descriptive sheets allow us to decide whether or not a sample of honey can be classified as belonging to one of these floral origins.

Keywords: Clover, descriptive sheets, Eucalyptus, honey, palynological, physicochemical properties, sensory.

Sonati F. Producción de miel en Argentina. Motivar 2017; 173: 23-6.
EU European Commission, Council Directive 2001/110/EC of 20 December 2001 relating to honey Official Journal of the European Communities 2002 http://eur-lex.europa.eu/legal-content/EN/ TXT/?uri=LEGISSUM:l21124a
Persano Oddo L, Piro R. Main European unifloral honeys: descriptive sheets. Apidologie (Celle) 2004; 35: S38-81.
Giorgi A, Madeo M, Baumgartner J, Lozzia GC. The relationships between phenolic content, pollen diversity, physicochemical information and radical scavenging activity in honey. Molecules 2011; 16(1): 336-47.
[http://dx.doi.org/10.3390/molecules16010336] [PMID: 21217600]
Khalafi R, Hossein Goli SA, Behjatian A. Characterization and classification of several monofloral Iranian honeys based on physicochemical properties and antioxidant activity. Int J Food Prop 2016; 19: 1065-79.
Ciappini MC, Vitelleschi M, Calviño AM. Chemometrics classification of Argentine clover and eucalyptus honeys according to palynological, physicochemical, and sensory properties. Int J Food Prop 2016; 19: 111-23.
Piana L, Persano Oddo L, Bentabol A, Bruneau E, Bogdanov S, Guyot-Declerck C. Sensory analysis applied to honey: state of the art. Apidologie (Celle) 2004; 35(Suppl. 1): S26-37.
Castro Vázquez L, Díaz Maroto MC, González Viñas MA, Pérez Coello MS. Differentiation of monofloral citrus, rosemary, eucalyptus, lavender, thyme and heather honeys based on volatile composition and sensory descriptive analysis. Food Chem 2009; 112: 1022-30.
Lenhardt L, Zeković I, Dramićanin T, Dramićanin MD, Bro R. Determination of the botanical origin of honey by front-face synchronous fluorescence spectroscopy. Appl Spectrosc 2014; 68(5): 557-63.
[http://dx.doi.org/10.1366/13-07325] [PMID: 25014599]
Shafiee S, Minaei S, Moghaddam-Charkari N. Ghasemi Var-namkhasti M, Barzegar M. Potential application of machine vision to honey characterization. Trends Food Sci Technol 2013; 30: 174-7.
Zakaria A, Shakaff AY, Masnan MJ, et al. A biomimetic sensor for the classification of honeys of different floral origin and the detection of adulteration. Sensors (Basel) 2011; 11(8): 7799-822.
[http://dx.doi.org/10.3390/s110807799] [PMID: 22164046]
Ciappini MC, Vitelleschi MS. Palynological characteristics of eucalyptus (Eucalyptus sp.) and clover (Trifolium sp.) honeys from Argentinean Pampean phytogeographic province. Rev Fac Cs Agrarias 2013; 45: 247-58.
Fagúndez GA, Caccavari MA. Pollen analysis of honeys from the central zone of the Argentine province of Entre Ríos. Grana 2006; 45: 305-20.
Loveaux J, Maurizio A, Vorwohl G. Methods of Melissopalynology. Bee World 1978; 59: 139-57.
Valle A, Andrada A, Aramayo E, Gil M, Lamberto S. A melissopalynological map of the South and Southwest of the Buenos Aires province, Argentina. Span J Agric Res 2007; 5: 172-80.
Von Der Ohe W. PersanoOddo L, Piana L, Morlot M, Martin P. Harmonized methods of melissopalynology. Apidologie (Celle) 2004; 35: S18-25.
AOAC. Official Methods of Analysis. 16th ed. Gaithersburg, MD: AOAC International 1995.
Ciappini MC, Stoppani F. Determination of antioxidant capacity, flavonoids, and total phenolic content in eucalyptus and clover honeys. J Apic Sci 2014; 58: 103-11.
Ciappini MC. Sensory analysis applied to bee honey In recent contributions to sensory analysis of foods. Kerala, India: Research Signpost 2013; pp. 21-32.
Ciappini MC, Di Vito MV, Gatti MB, Calviño AM. Development of a quantitative descriptive sensory honey analysis: application to eucalyptus and clover honeys. Adv J Food Sci Technol 2013; 5: 829-38.
Malacalza N, Caccavari MA, Fagúndez L, Lupano CE. Unifloral honeys of the province of Buenos Aires, Argentine. J Sci Food Agric 2005; 85: 1389-96.
Terrab A, Díez MJ, Heredia F. Palynological, physico-chemical and colour characterization of Moroccan honeys: I. River red gum (Eucalyptus camaldulensis Dehnh) honey. Int J Food Sci Technol 2003; 38: 379-86.
Seijo MC, Aira MJ, Méndez J. Palynological differences in the pollen content of eucalyptus honey from Australia, Portugal and Spain. Grana 2003; 42: 183-90.
Basualdo M, Pereda A, Bedascarrasbure E. Caracterización botánica y geográfica de mieles de la cuenca del Salado, provincia de Buenos Aires, Argentina. RIA 2009; 35: 5-14.
Burgos MG, Sánchez AC. Preferencias alimenticias en las mieles inmaduras de Apis mellífera en el Chaco Serrano (Jujuy, Argentina). Bol Soc Argent Bot 2014; 4: 41-50.
Salgado C, Maidana JF. Physicochemical characterisation of honey produced in the Chaco province (Argentina). Rev Fac Cs Agrarias UNCu 2014; 46: 191-201.
Abell D, Friebe H, Schweger C, Kwok A, Sporns P. Comparison of processed unifloral clover and canola honey. Apidologie (Celle) 1996; 27: 451-60.
Gaggiotti MC, Signorini M, Sabbag N, Wanzenried R, Cuatrín A. Honey produced in a dairy farming system based on alfalfa (physicochemical, palynological and sensorial analyse). Arch Latinoam Prod Anim 2014; 22: 15-20.
Stand Codex. Codex Alimentarius Comission 2001.
CAA. Reglamento Técnico MERCOSUR de Identidad y Calidad de Miel GMC- Res Nº 015/94, 1994; Incorporada por Resolución MSyASN° 003. Boletín Oficial 1994.
Bogdanov S. Contaminants of bee products. Apidologie (Celle) 2006; 37: 1-18.
Baldi B. La miel: una mirada científicaEntre Ríos, Argentina:Eduner 2010; p 079.
El Sohaimy AS, Masry SHD, Shehata MG. Physicochemical characteristics of honey from different origins. Ann Agric Sci 2015; 60: 279-87.
Silvano MF, Varela MS, Palacio MA, Ruffinengo S, Yamul DK. Physicochemical parameters and sensory properties of honeys from Buenos Aires region. Food Chem 2014; 152: 500-7.
[http://dx.doi.org/10.1016/j.foodchem.2013.12.011] [PMID: 24444967]
Makhloufi C, Kerkvliet J, RicciardelliD’Albore G, Choukri A, Samar R. Characterization of Algerian honeys by palynological and physico-chemical methods. Apidologie (Celle) 2010; 41(5): 509-21.
Serra Bonvehí J. A study of physicochemical properties of eucalyptus honey in Spain. Anales de Bromatología 2001; 41: 41.
UNI U5900642 Ente Nazionale Italiano di Unificazione Milano,Italy 2010.
Nocentini D, Guarnieri M, Soligo C. Nectardefense and hydrogenperoxide in floral néctar of Cucurbita pepo. Acta Agrobot 2015; 68(2): 187-93.
Carter C, Thornburg RW. Is the nectar redox cycle a floral defense against microbial attack? Trends Plant Sci 2004; 9(7): 320-4.
[http://dx.doi.org/10.1016/j.tplants.2004.05.008] [PMID: 15231276]
Draiaia R, Chefrour A, Dainese N, Manzinello C, Gallina A, Mutinelli F. Physicochemical parameters and antibiotic residual in Algerian honey. Afr J Biotechnol 2015; 14: 1242-51.
Hamdy AA, Ismail HM. El-Moneim A Determination of flavonoid and phenolic acid contents of clover, cotton and citrus floral honeys. J Egypt Public Health Assoc 2009; 84: 3-4.
Orantes Bermejo J, Gomez Pajuelo A. Mieles monoflorales de la Península Ibérica. Granada, España: Asoc. Envasadores de Miel de España y Consejo Regulador D.O.P. Miel de Granada 2007.
Ferreira ICFR, Aires E, Barreira JCM, Estevinho LM. Antioxidant activity of Portuguese honey samples: different contributions of the entire honey and phenolic extract. Food Chem 2009; 114: 1438-43.
Vit P, Gutiérrez MG, Titera D, Vendar M, Rodríguez Malaver AJ. Mieles checas categorizadas según su actividad antioxi-dante. Acta Bioq Clin 2008; 42: 237-44.
Can Z, Yildiz O, Sahin H, Akyuz Turumtay E, Silici S, Kolayli S. An investigation of Turkish honeys: their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chem 2015; 180: 133-41.
[http://dx.doi.org/10.1016/j.foodchem.2015.02.024] [PMID: 25766810]
González Lorente M, De Lorenzo Carretero C, Perez Martin R. Sensory attributes and antioxidant capacity of Spanish Honeys. J Sens Stud 2008; 23: 293-302.
Meda A, Lamien C, Romito M, Millongo J, Nacoulma O. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavening activity. Food Chem 2005; 91: 571-7.
Escuredo O, Dobre I, Fernández-González M, Seijo MC, Seijo Coello MC. Contribution of botanical origin and sugar composition of honeys on the crystallization phenomenon. Food Chem 2014; 149: 84-90.
[http://dx.doi.org/10.1016/j.foodchem.2013.10.097] [PMID: 24295680]
Vela L, De Lorenzo C, Pérez RA. Antioxidant capacity of Spanish honeys and its correlation with polyphenol content and other physicochemical properties. J Sci Food Agric 2007; 87: 1069-75.
Rodríguez A, Pérez E, Vit P. Capacidad antioxidante de mieles venezolanas de los géneros Apis, Melipona y Tetragonisca, evaluados por tres métodos. Rev Inst Nac Higiene Rafael Rangel 2007; 38: 30-4.
Montenegro G, Pizarro R, Mejías E, Rodríguez S. Evaluación biológica de polen apícola de plantas nativas de Chile. Phyton 2013; 82: 7-14.
Bastos DHM, Franco MB, Da Silva MAAP, Janzantti NS, Marques MO. Composição de voláteis e perfil de aroma e sabor de méis de eucalipto e laranja. Food Sci Technol (Campinas) 2002; 22: 122-9.
Bogdanov S, Gallman P. Authenticity of honey and other bee products: state of the art. ALP Science 2008; 520: 2-12.

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Article Details

Year: 2020
Page: [919 - 927]
Pages: 9
DOI: 10.2174/1573401314666180723161102
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