Background: The need to enhance the stability of proteins is vital to protein engineering
and design. The manipulation of protein stability is also important to understand the principles that
govern protein thermostability, both in basic research and industrial application.
Objective: To build models that can discriminate thermophilic and mesophilic proteins and comprehend
the factors influencing protein thermostability using machine learning methods.
Method: A total of 613 protein features were calculated and various feature selection algorithms were
used to build subset features. Support vector machine and decision tree methods were applied to predict
the thermostability of the proteins, and the problems caused by unbalanced data were resolved by
using a grid search method to find the best weights of error costs for different classes.
Results: According to the result, the influence of primary structure on the thermostability of a protein
was more important than the influence of secondary structure. The best classification model was obtained
when the support vector machine was run on the subset of amino acid composition plus amino
acid class composition, which yielded a prediction accuracy of 84.07%. At the primary structure level,
Gln, Glu, and Ser were the features that contributed most to protein thermostability. At the secondary
structure level, Q_coil and Helix_E were the most important features affected protein thermostability.
Conclusion: These results suggested that the thermostability of a protein was mainly associated with
the primary structural features of the protein.