PCR Amplification Strategies Towards Full-length HIV-1 Genome Sequencing

Author(s): Chao Chun Liu, Hezhao Ji*

Journal Name: Current HIV Research

Volume 16 , Issue 2 , 2018


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Graphical Abstract:


Abstract:

The advent of next-generation sequencing has enabled greater resolution of viral diversity and improved feasibility of full viral genome sequencing allowing routine HIV-1 full genome sequencing in both research and diagnostic settings. Regardless of the sequencing platform selected, successful PCR amplification of the HIV-1 genome is essential for sequencing template preparation. As such, full HIV-1 genome amplification is a crucial step in dictating the successful and reliable sequencing downstream. Here we reviewed existing PCR protocols leading to HIV-1 full genome sequencing. In addition to the discussion on basic considerations on relevant PCR design, the advantages as well as the pitfalls of the published protocols were reviewed.

Keywords: HIV-1, Full genome, PCR, Methods, Sequencing, Next generation sequencing.

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Article Details

VOLUME: 16
ISSUE: 2
Year: 2018
Published on: 15 August, 2018
Page: [98 - 105]
Pages: 8
DOI: 10.2174/1570162X16666180626152252
Price: $65

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