Background: Plants of the genus Campomanesia belong to the family Myrtaceae and are
very abundant in Cerrado areas. Teas from leaves of species of this genus are used for intestinal infections,
combating obesity, stomach pathology, fever and among others.
Objective: The present study evaluated the chemical composition and antioxidant potential, cytotoxicity
and genotoxicity of ethanolic extract from leaves of Campomanesia pubescens (Mart. ex DC.) O.Berg.
Method: For the evaluation of antioxidant activity, the free radical DPPH and for determination of
phenolic compounds Folin-Ciocalteau reagent were used. Identification of the substances was performed
by HPLC-DAD by comparison of the retention times with standards analyzed under the same
conditions and by evaluation of molecular absorption spectra in the ultraviolet and visible region. The
cytotoxicity, genotoxicity were evaluated using Allium cepa bioassay.
Results: In the ethanolic extract 7-hydroxy-6-methyl-5-methoxyflavanone, 5,7-dihydroxy-6-methylflavanone,
5,7-dihydroxy-8-methylflavanone, 2´,4´-dihydroxy-6´-methoxychalcone, 5,7-dihydroxy-6,8-
dimethylflavanone, 2´,4´-dihydroxy-5´-methyl-6´-methoxychalcone and 2´,4´-dihydroxy-3´,5´-dimethyl-6´-
methoxychalcone were identified. The extract showed antioxidant activity and cytotoxic effects on cell
division and increased chromosomal alterations in Allium cepa test.
Conclusion: These results showed antioxidant activity and suggest the cytotoxic and genotoxic effects
in Allium cepa of ethanolic extract obtained from the leaves of Campomanesia pubescens.