Background: The photosynthetic unicellular microalgal strain, Chlorella vulgaris is
considered as a robust biomass and lipid producer for human food and feed and also green energy
production. The culture media composition plays a crucial role in biomass and lipid production and
Methods: The photosynthetic green microalga C. vulgaris was studied in three distinct cultivation
experiments each for 22 days, using BG11 and f/2 culture media to investigate the effects of different
levels of nitrogen and culture media replacement on cell growth, lipid production and productivity.
Results: In f/2 culture medium, the total lipid production reached was 0.246 g L-1 (41.55%) which
was 147% higher than its counterpart in BG11 medium (0.298 g L-1). On the other hand, the highest
biomass (1.492 g L-1) was attained in BG11-3 medium with 6.00 g nitrate L-1 and this was correlated
with higher total chlorophyll content (15.189 mg L-1) and cell number (3.812 × 106 cell mL-1).
A fatty acid profile, composed of saturated, monounsaturated and polyunsaturated fatty acids was
detected and included stearic acid, oleic acid, α-linolenic acid and γ-linolenic acid which have nutritional
or combustion importance.
Conclusion: These data suggest that this C. vulgaris strain may be considered as a robust source for
biomass, lipid and green energy production. The newly established patents have shed light on the
future directions to achieve higher lipids and fatty acids with improved properties through different
bioprocess and genetic engineering approaches.