Title:Bursal Hexapeptide, A Potential Immunomodulator, Inhibits Tumor Cells Proliferation via p53 Signaling Pathway
VOLUME: 18 ISSUE: 11
Author(s):Cong Zhang, Jiangfei Zhou, Shengnan Li, Kairui Cai, Xiangling Guo, Chengshui Liao* and Chen Wang*
Affiliation:Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang, Key Lab of Veterinary Oncological Immunology, Henan University of Science and Technology, Luoyang
Keywords:The bursa of Fabricius, bursal hexapeptide, gene microarrays, antiproliferation, p53 signaling pathway, immunomodulator.
Abstract:Background: The Bursa of Fabricius (BF) is acknowledged as the central humoral immune organ
unique to birds. Bursal Hexapeptide (BHP, AGCCNG) is a recently reported bursal-derived bioactive peptide.
However, there are few reports of the molecular basis of the mechanism on immune induction and potential
antitumor activity of BHP.
Method: In this paper, Gene microarray analyses demonstrated that BHP regulated expression of 1347 genes,
of which 832 were up-regulated and 515 were down-regulated. Differentially expressed genes involved in
various pathways were identified, of which 16 pathways were associated with immune responses and tumorigenic
processes.
Result: Specifically, we found that BHP selectively inhibited tumor cell proliferation. Furthermore, BHP
enhanced antitumor factor p53 luciferase activity and stimulated expression of p53, p21, and p130 protein.
Moreover, we observed that the inhibitory effect of BHP on cell proliferation and premature senescence in a
p53-dependent manner.
Conclusion: Taken together, we uncovered that BHP may be involved in antitumor suppressor via p53 signaling
pathway.
