Background: Antimicrobial drug resistance is an emerging problem, which leads to a failure
in the control of infectious diseases thereby, adversely affecting patient care and reducing effective
management of infectious diseases globally. Thus, search for new and more effective alternatives is
needed. Daphne retusa Hemsl. (Daphne) has medicinal values and is reported to be widely used in curing
a variety of human ailments.
Objective: Current study assesses in-vitro antimicrobial activity of the crude extract of D.retusa (whole
plant) and its derived fractions against clinically isolated human pathogenic bacteria and fungi.
Materials and Methods: Whole plant of D.retusa was powder dried and then extracted with methanol
(E1). The resultant was fractionated to give Chloroform fraction (E2), Butanol fraction (E3) and Ethyl
acetate fraction (E4). The crude extract and derived fractions were assessed for antimicrobial and antifungal
activity by using agar well diffusion method and their MICs were found following Clinical and
Laboratory Standard Institute (CLSI) guidelines.
Result: Our study shows that D.retusa has very good inhibitory action against different bacterial and
fungal strains. All of the extracts were active against almost every microorganism used in the study.
E2 has the maximum percent of inhibition against bacterial growth while E1 has themaximum percent of
inhibition against fungal growth. Streptococcus pneumonia was the most susceptible bacteria while
among fungi, Gongronella butleri showed highest susceptibility.
Conclusion: Results justify the use of D. retusa in the treatment of microbial infections. For the development
of a novel antibiotic, the crude extract and its derived fractions need further exploration; with
emphasis to isolate and identify the active constituents that are responsible for antibacterial and antifungal