Title:125I-Insulin and 125I-Insulinlike Growth Factor-I in Chicken Hepatocytes Internalization. The Peptides Distribution and Action in Birds
VOLUME: 12 ISSUE: 2
Author(s):A.P. Kolychev* and E.E. Ternovskaya
Affiliation:Laboratory of Enzymes Comparative Biochemistry, Sechenov Institute of Evolutionary Physiology and Biochemistry (Russian Academy of Sciences), Saint-Petersburg, Biophysics Department, Biology Faculty, Saint-Petersburg State University, Saint-Petersburg
Keywords:Insulin, IGF-I, receptor, endocytosis, internalization, temperature, hepatocytes, phylogenesis.
Abstract:Background: 125I-insulin and 125I-IGF-I distribution during internalization is seemingly a key
condition that determines hormone functional role in a given organism. Endocytosis course of 125Iinsulin
and 125I-IGF-I in isolated chicken hepatocytes occurred in different ways as compared with rat
hepatocytes. 125I-insulin and 125I-IGF-I distribution across cell membrane during internalization was different
in bird cells.
Objective: Analysis of their distribution across the cell membrane in chicken cells.
Methods: Hepatocytes were isolated according to Berry and Friend (with modifications). Hormone/
receptor complex internalization course was evaluated in a separate experiment with 125I-insulin
or 125I-IGF-I. It included a four steps procedure: cells preincubation with labelled ligands at 6°C, incubation
at 12°C and 37°C to activate internalization; two-stage cell washing with another buffer, and lastly,
radioactivity was counted stepwise in first and second precipitates.
Results: 125I-insulin and 125I-IGF-I distribution across cell membrane during internalization into chicken
hepatocytes considerably differed from the same peptides distribution in rat hepatocytes. In chicken
hepatocytes, 125I-insulin distribution, temperature dependent in a variable manner, with clear “intracellular”
type distribution at 6°C, and stable, resistant to changes in incubation temperature, “membrane”
type of 125I-IGF-I distribution were revealed.
Conclusions: 125I-insulin and 125I-IGF-I distribution is a specific characteristic of internalization, which
is capable of changing, despite retaining basic internalization characteristics of both insulin and IGF-I in
the cells of different animal species. The differences of 125I-insulin and 125I-IGF-I distribution types in
chicken and rat cells, allow us to guess correctly other functional roles of insulin and IGF-I in birds as
compared with mammals.
