Background: Drug-induced Liver Injury (DILI) is an important cause of acute liver failure cases in the
United States, and remains a common cause of withdrawal of drugs in both preclinical and clinical phases.
Methods: A structured search of bibliographic databases – Web of Science Core Collection, Scopus and Medline for
peer-reviewed articles on models of DILI was performed. The reference lists of relevant studies was prepared and a
citation search for the included studies was carried out. In addition, the characteristics of screened studies were described.
Results: One hundred and six articles about the existing knowledge of appropriate models to study DILI in vitro and
in vivo with special focus on hepatic cell models, variations of 3D co-cultures, animal models, databases and predictive
modeling and translational biomarkers developed to understand the mechanisms and pathophysiology of DILI
Conclusion: Besides descriptions of current applications of existing modeling systems, associated advantages and
limitations of each modeling system and future directions for research development are discussed as well.