Simultaneous Determination of Phenolic Acids, Anthraquinones, and Flavonoids in the Aerial Part and the Fruit of Xanthium Sibiricum by LC- ESI- QTRAP-MS/MS

Author(s): Hui Zhao, Hao Cai, Juan-Xiu Liu, Sheng-Nan Wang, Xun-Hong Liu*, Ying Yan, Li-Si Zou, Yu-Jiao Hua

Journal Name: Current Pharmaceutical Analysis

Volume 15 , Issue 5 , 2019


Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Abstract:

Background: Xanthium sibiricum is a well-known traditional Chinese medicine (TCM) that has been commonly used to treat rhinitis and related nasal diseases. The aim of this study was to develop a comprehensive analytical method based on high-performance liquid chromatographyelectrospray ionization coupled with triple quadrupole-linear ion trap mass spectrometry (LC-ESIQTRAP- MS/MS) for the simultaneous determination of phenolic acids, anthraquinones, and flavonoids in the aerial part and fruit of Xanthium sibiricum.

Methods: The separation was completed on Agilent ZORBAX SB-C18 column (250 × 4.6 mm, 5μm) using methanol and 0.2% (v/v) aqueous formic acid as the mobile phase. The target components were analyzed in negative ion mode with accurate and sensitive multiple reaction monitoring (MRM) mode.

Results: The correlation coefficients of all the calibration curves were higher than 0.9994. Relative standard deviations of intra- and inter-day precisions of the eighteen components were all lower than 2.87% and the recoveries were in the range from 97.73% to 101.82%. The validated method was successfully applied to possess forty Xanthium sibiricum samples (Xanthii Herba, Xanthii Fructus, and processed Xanthii Fructus) collected from different places in P. R. China. Furthermore, principal component analysis (PCA) was performed to evaluate and classify the samples according to the contents of the eighteen bioactive components.

Conclusion: All the results demonstrated that the developed method was useful and could be applied for the overall assessment of the quality of Xanthii Herba and Xanthii Fructus.

Keywords: Xanthium sibiricum, LC-ESI-QTRAP-MS/MS, phenolic acids, anthraquinones, flavonoids, PCA.

[1]
Dai, Y.H.; Cui, Z.; Wang, D.; Li, J.L. Chemical constituents from the fruits of Xanthium sibiricum. J. Shenyang Pharm. Univ., 2008, 25(8), 630-632.
[2]
Lei, Y.; Li, W.D.; Cai, B.C. Research progress of Xanthium sibiricum. Res. Pract. Chin. Med, 2011, 25(4), 81-84.
[3]
Su, X.G.; Mi, S.Q.; Wang, N.S.; Huang, T.L. Pharmaceutical Research progress of Xanthium sibiricum. Tradit. Chin. Drug Res. Pharmacol, 2006, 17(1), 68-72.
[4]
Ruan, G.H.; Li, G.K. Research progress on chemical composition, separation, and analysis of Xanthium sibiricum. Chin. Tradit. Pat. Med., 2008, 30(3), 421-426.
[5]
Yang, L.; Wu, J.X.; Xu, S.J.; Su, Z.J.; Zeng, X.; Li, X.; Chen, L.L. Characterization of phenolic acids and quantitative analysis of chlorogenic acid contained in Fructus Xanthii. Chin. J. Exp. Tradit. Med. Form, 2011, 17(19), 85-88.
[6]
Huang, W.H.; Yu, J.G.; Sun, L.; Guo, B.L.; Basang, D.J.; Li, D.Y. Study on chemical constituents of Chinese medicine Xanthium sibiricum. Chin. J. Chin. Mater. Med, 2005, 30(13), 1027-1028.
[7]
Han, T.; Li, H.L.; Hu, Y.; Zhang, Q.Y.; Huang, B.K.; Zheng, H.C.; Rahman, K.; Qin, L.P. Phenolic acids in Fructus Xanthii and determination of contents of total phenolic acids in different species and populations of Xanthium in China. J. Chin. Integr. Med., 2006, 4(2), 194-198.
[8]
Song, L.L.; Sha, J.Q.; Zhao, H.; Zhao, Y. Determination of TPA content of Xanthium sibiricum Patrin exWidder and anti-inflammatory effect. Lishizhen Med. Mater. Med. Res., 2013, 24(10), 2331-2332.
[9]
Cao, L.; Zhou, J.J. Research progress of anthraquinone compounds. Northwest Pharm. J., 2009, 24(3), 237-238.
[10]
Zhong, D.C.; Zhang, S.F.; Chen, Z.F.; Wang, H.S.; Liang, H. Research progress on the extraction and purification of natural flavonoides and their metal complexes. Chem. World, 2006, 47(9), 561-564.
[11]
Wang, R.Y.; Liu, X.F.; Liu, M.H.; Lv, W.Y. Study on the antioxidant activity and the determination of total flavonoids of Fyutus xanthii extracts. Yunnan J. Tradit. Chin. Med. Mater. Med, 2008, 29(9), 42-43.
[12]
Tong, J.T.; Xu, S.Q.; Li, W.D.; Lei, Y. Determination of chlorogenic acid in Xanthium sibiricum by HPLC. Chin. J. Hosp. Pharm, 2011, 31(20), 1739-1740.
[13]
Ming, Q.L.; Gao, X.; Zhang, H.; Qin, L.P.; Han, T. Determination of chlorogenic acid in three species of cocklebur fruits from different regions of China by HPLC method. Pharm. Care Res, 2008, 8(4), 258-260.
[14]
Wang, H.; He, G.X.; Yao, Y. Determination of total anthraquinones in Fructus Xanthii. Chin. J. Mod. Drug Appl, 2009, 3(1), 89-90.
[15]
Yin, J.X.; Deng, X.H.; Che, X.Y.; Zhang, L.H. Study on TLC identification of Fructus Xanthii. West China J. Pharm. Sci.,, 2005, 20(1), 67-69.
[16]
Li, G.H.; Zheng, X. Optimization of ultrasonic-assisted extraction of total flavonoids from Fructus Xanthii by response surface methodology. J. Northwest Univ. Nationalities. Nat. Sci., 2015, 36(1), 53-58.
[17]
Zhang, C.; Zhu, K.Y.; Zhang, P.W.; Ye, H.Y.; Shi, M.M.; Qi, X.; Liu, Q.S. The study on determination content of flavonoids extracts from Fructus Xanthii and Hypericum japonicum thunb. Chin. Agric. Sci. Bull, 2014, 30(6), 306-313.
[18]
Tian, J.; Xia, Y.F.; Fang, K.H. Simultaneous determination of eight phenolic acids in Xanthium sibiricum medicinal herbs by HPLC. J. Chin. Med. Mater.,, 2013, 36(10), 623-1626.
[19]
Liu, J.X.; Luo, Y.Y.; Liu, X.H.; Song, J.P.; Hou, Y.; Ma, Y.; Hua, Y.J.; Cai, B.C. Simultaneous determination of seven phenolic acids from Xanthii Herba and Xanthii Fructus by HPCE. Chin. Pharm. J, 2015, 50(4), 366-370.
[20]
Duo, R.; Liu, Y.H.; Wang, M.K.; Yi, J.H. HPLC simultaneous determination of the content of 6 constituents in Fructus Xanthii. Chin. J. Pharma. Anal, 2013, 33(1), 78-82.
[21]
Yang, L.; Su, Z.J.; Xu, S.J.; Wu, J.X.; Chen, L.L.; Zhou, R.L.; Li, X.; Zeng, X. Simultaneous determination of phenolic acids in Cangerzi by ultra-performance liquid chromatography. Acta Pharm. Sin.,, 2010, 45(12), 1537-1540.
[22]
Hong, Y.; Han, Y.Q.; Xia, L.Z.; Gui, J.; Chen, X.; Sun, Y.H. Simultaneous determination of nine phenolic acid components in Xanthii Fructus. Chin. Pharm. J.,, 2013, 48(13), 1109-1112.
[23]
Qin, S.L.; Ma, L.H.; Wang, Z.Z.; Chen, B.; Xie, D.H.; Jia, X.B. Simultaneous determination of five kinds of phenolic acids in stir-fried Fructus Xanthii formula granule by UPLC. Chin. Tradit. Pat. Med, 2012, 34(7), 1393-1395.
[24]
Xiang, Y.; Li, L.J.; Abliz, Z. Application of LC-MS in the analysis of constituents of crude plant extracts. Acta. Pharm. Sin, 2002, 37(5), 389-395.
[25]
Harmita1; Suryadi, H.; Setiawati, A.; Damayanti, D. Development and validation of a method for simultaneous quantification of seven water-soluble vitamins in pediatrics syrup by UPLC-MS/MS. Curr. Pharm. Anal., 2018, 14(1), 23-34.
[26]
Shi, X.W.; Zhang, K.; Xue, N.; Su, L.F.; Ma, G.X.; Qi, J.L.; Wu, Y.B.; Wang, Q.; Shi, Q.W. Differentiation of genuine Inula britannica L. and substitute specimens based on the determination of 15 components using LC-MS/MS and principal components analysis. Food Chem., 2013, 141(4), 4019-4025.
[27]
Napolitano, A.; Akay, S.; Mari, A.; Bedir, E.; Pizza, C.; Piacente, S. An analytical approach based on ESI-MS, LC-MS and PCA for the quali-quantitative analysis of cycloartane derivatives in Astragalus spp. J. Pharm. Biomed. Anal., 2013, 85(4), 46-54.
[28]
Su, J.; Yuan, Z.F.; Zhang, L.T.; Wang, C.Y.; Lv, C.Y.; Liu, W.N. Determination of stilbene glycoside and anthraquinones in Radix Polygoni Multiflori and Caulis et Folium Polygoni Multiflori by RP-HPLC. Chin. Tradit. Herbal Drugs, 2007, 38(2), 278-281.
[29]
Qiao, L.P.; Fu, Y.; Ye, X.Q.; Sun, Y.J. Recent Advances of phenolic acid bioactivities. J. Chin. Inst. Food Sci. Techno, 2013, 13(10), 144-152.
[30]
Rao, C.; Huang, X. Advances in research on the common mechanisms of anti-inflammatory activity and antitumor activity by flavonoids. Strait Pharm. J., 2010, 22(6), 8-11.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 15
ISSUE: 5
Year: 2019
Published on: 22 May, 2019
Page: [542 - 553]
Pages: 12
DOI: 10.2174/1573412914666180502125549
Price: $65

Article Metrics

PDF: 49
HTML: 2