Abstract
Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.
Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China.
Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549.
Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.
Keywords: SOS/umu tests, high-content screening, in vitro micronucleus tests, cytotoxicity, genotoxicity, tannery chemicals.
Combinatorial Chemistry & High Throughput Screening
Title:In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests
Volume: 21 Issue: 4
Author(s): Zehao Huang, Na Li, Kaifeng Rao, Cuiting Liu, Zijian Wang*Mei Ma*
Affiliation:
- State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing,China
- University of Chinese Academy of Sciences, College of Resources and Environment, Beijing,China
Keywords: SOS/umu tests, high-content screening, in vitro micronucleus tests, cytotoxicity, genotoxicity, tannery chemicals.
Abstract: Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.
Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China.
Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549.
Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.
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Cite this article as:
Huang Zehao , Li Na , Rao Kaifeng , Liu Cuiting , Wang Zijian *, Ma Mei *, In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests, Combinatorial Chemistry & High Throughput Screening 2018; 21 (4) . https://dx.doi.org/10.2174/1386207321666180330120248
DOI https://dx.doi.org/10.2174/1386207321666180330120248 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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