Title:In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests
VOLUME: 21 ISSUE: 4
Author(s):Zehao Huang, Na Li, Kaifeng Rao, Cuiting Liu, Zijian Wang* and Mei Ma*
Affiliation:State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, Beijing, Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, Beijing, The Third Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangzhou, State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, University of Chinese Academy of Sciences, College of Resources and Environment, Beijing
Keywords:SOS/umu tests, high-content screening, in vitro micronucleus tests, cytotoxicity, genotoxicity, tannery chemicals.
Abstract:Background: More than 2,000 chemicals have been used in the tannery industry.
Although some tannery chemicals have been reported to have harmful effects on both human health
and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.
Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used
in China.
Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with
Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were
determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell
lines MGC-803 and A549.
Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell
assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced
DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism.
Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging
effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural
feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the
chromosome-damaging effects of the four MN-test-positive chemicals.
