Background: SjOgren's Syndrome (SS) is a systemic and chronic autoimmune disorder
that affects the exocrine glands with massive autoantibody production. Although the pathogenesis
of the disorder is incompletely understood, but some studies have reported that anti-moesin antibodies
have been detected in autoimmune diseases with which SS is closely associated. Here, we
have investigated moesin's potential involvement in SS.
Objective: This study aims to verify whether moesin is a specific autoantigen involved in Chinese
Hans SS patients.
Methods: First, recombinant human moesin was expressed and purified. Next, the protein was verified
as antigen by Western blotting and immunoprecipitation. The positive protein band in the immunoprecipitation
was identified by (MALDI-TOF/TOF). Finally, an optimized ELISA (Enzyme-
Linked Immunosorbent Assay) kit was developed to measure the titer concentration of anti-moesin
antibody-positive patients in a large cohort of clinical subjects.
Results: Univariate analysis revealed that the proportion of individuals positive for serum IgG
against recombinant human moesin was 42 % in a group of Chinese Hans SS patients (21 of 50), 22
% in systemic lupus erythematosus patients and (11 of 50), compared to only 4 % in healthy controls
(2 of 50).
Conclusion: An association between anti-moesin antibodies and SS manifestation have been found
which may be considered a suspected serum biomarker for the development of SS.