Title:Mosquito Larvicidal and Antimicrobial Properties of Phyto-synthesized Silver Nanoparticle from Aqueous and Methanol Extracts of Wrightia tinctoria Leaves
VOLUME: 2 ISSUE: 2
Author(s):Sangilimuthu Alagar Yadav*, Nalini Madanogopal, Naveen Kumar S and M. Karpakavalli
Affiliation:Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore-641021, Tamil Nadu, PG & Research Department of Zoology, Poompuhar College, Melaiyur 609107, Tamil Nadu, Department of Biotechnology, Karpagam Academy of Higher Education, Coimbatore-641021, Tamil Nadu, Department of Pharmaceutical Chemistry, Karpagam College of Pharmacy, Coimbatore-641032, Tamil Nadu
Keywords:Wrightia tinctoria, silver nanoparticles, larvicidal activity, antimicrobial, phytomediated nano particle, Anopheles
stephensi.
Abstract:Objective: An eco-friendly, cost-effective mosquito larvicidal and antimicrobial silver nanoparticle
was produced from Wrightia tinctoria leaves.
Method: The green route synthesis of silver nanoparticles using aqueous and methanol extract of W.
tinctoria leaves and synthesized AgNPs were characterized by UV-Vis, FT-IR, EDAX, XRD and
SEM. The antimicrobial potentials of AgNPs using well diffusion method against human bacterial
pathogens and mosquito larvicidal activity were also observed on treatment with AgNPs using
mortality test and FT-IR spectral studies.
Results: The maximum absorbance was observed between 400 nm to 480 nm for the synthesized
AgNPs, the synthesized AgNPs were spherical shaped with size ranging between 50-90 nm. The
EDAX spectrum clearly showed a strong peak for silver at 3 keV. The AgNPs showed antibacterial
potential against clinical pathogens such as Escherichia coli, Pseudomonas aeruginosa, Pseudomonas
fluorescens, Proteus vulgaris and Staphylococcus aureus and inhibited the mosquito larval growth as
parricidal agent. Thus an eco-friendly and cost-effective production of antimicrobial and parricidal
AgNPs from W. tinctoria leaves will enable biocontrol of mosquitoes.
