Title:Determination of Parameters of Oxidative Stress in vitro Models of Neurodegenerative Diseases-A Review
VOLUME: 13 ISSUE: 2
Author(s):Chistiane Mendes Feitosa*, George Laylson da Silva Oliveira, Antonio do Nascimento Cavalcante, Soane Kaline Morais Chaves and Mahendra Rai
Affiliation:Department of Chemistry, University Federal of Piaui, Teresina, Department of Pharmacy, University Federal of Piaui, Teresina, Department of Chemistry, University Federal of Piaui, Teresina, Department of Pharmacy, University Federal of Piauí, Teresina, Department of Biotechnology, Sant Gadge Baba Amravati University Amravati- 444 602, Maharashtra
Keywords:Alzheimer’s disease, parkinson, huntington, amyotrophic lateral sclerosis, neurodegenerative diseases, oxidative
stress.
Abstract:Oxidative stress is a major mechanism underlying the development of various neurodegenerative
diseases (Alzheimer, Parkinson, Huntington and amyotrophic lateral sclerosis). Excessive
formation of reactive oxygen species (ROS) and nitrogen (RNSs) can overburden the ability of
the enzymatic antioxidant defense mechanisms (superoxide dismutase, catalase and glutathione
reductase) and non-enzymatic (uric acid, ascorbic acid, α-tocopherol and reduced glutathione),
causing the development of oxidative stress, and consequently, impairing the neuronal system cells
by means of oxidative damage to a variety of important biological molecules such as lipids, DNA
and proteins. Considering the importance of oxidative stress in neurodegenerative diseases, the
present review aims to address the main parameters evaluated in in vitro studies on oxidative stress
in different models of neurodegenerative diseases.The literary review was conducted through Pubmed,
Science Direct, LILACS, Scielo and Google using following keywords: oxidative stress, neurodegenerative
diseases and parameters of oxidative stress. We selected articles published between
2002 and 2017.The in vitro evaluation of the oxidative stress related parameters has provided a
preliminary view about the pathogenesis of many neurodegenerative diseases (Alzheimer's, Parkinson's,
Huntington's and Amyotrophic lateral sclerosis). In this way, it has demonstrated the mechanism
of action of ROS/RNSs in these diseases by direct or indirect detection through several experimental
procedures in vitro.
