Objective: The current work was attempted to formulate and evaluate the topical sustained
release delivery systems called cubosomes containing Tretinoin for the acne treatment. The recent patents
on various formulations of tretinoin (EP0408370A2) helped in selecting a new formulation and
Methods: Tretinoin loaded cubosomes were prepared by bottom-up technique, using varying the concentration
of lipid and surfactant and keeping the drug concentration constant, a total of nine formulations
of tretinoin was developed. These preparations were evaluated for surface charge, particle size,
particle morphology, encapsulation efficiency, in-vivo and in-vitro release studies of gel enriched with
cubosome dispersion. Finally, the stability studies of cubosomal gel were performed on optimized
Results: Significant result were obtained with tretinoin formulation as the drug is lipophilic, so it gives more depot
effect on the epidermis and good retention property. The data obtained from the formulations showed that formulation
TCF-5 was the optimized formulation which exhibited better drug release and entrapment efficiency.
Conclusion: It can be concluded that cubosomes offer benefits of quick onset as well as the maximal release
of drug with fewer side effects. Thus, cubosomes represent a capable transporter having the property
to sustain the release of drug, potential to localize the drug in the skin with a possible clinical application
for acne vulgaris treatment due to cubosome depot effect on the epidermis.