Title:Investigating the Association Between miR-608 rs4919510 and miR-149 rs2292832 with Colorectal Cancer in Iranian Population
VOLUME: 7 ISSUE: 2
Author(s):Reza Ranjbar, Vahid Chaleshi, Hamid Asadzadeh Aghdaei and Saeid Morovvati*
Affiliation:Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Human Genetics Research Center, Baqiyatallah University of Medical Sciences (BMSU), Tehran
Keywords:Colorectal cancer, miR-146, miR-608, polymorphism, rs2292832, rs4919510, single nucleotide.
Abstract:Background: Single Nucleotide Polymorphisms (SNPs) in microRNA (miRNA) networks
may serve as diagnostic and prognostic biomarkers of a variety of diseases such as cancer. Some studies
have been performed to examine associations between miR-149 and miR-608 polymorphisms and
susceptibility to colorectal cancer, but the results remain controversial and race-dependent.
Objective: The aim of our study was to investigate the association of miR-608 (rs4919510) and miR-
149 (rs2292832) with colorectal cancer and its clinical features in a sample of Iranian population.
Methods: This retrospective study was conducted on 76 CRC cases and 70 controls. Genotyping was
performed using the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP)
method. To confirm the RFLP process, 10% of the PCR products were validated by direct sequencing.
Results: Our findings showed significant correlation between adjusted data of rs2292832 with sex and
age in TT genotype (OR= 5.148, 95% CI=1.081 ± 24.511, P=0.04). Distribution of rs4919510 polymorphism
was not significantly different between controls and patients (CG, adjusted OR= 1.243,
95% CI=0.546 ± 2.831; P=0.604 and GG, adjusted OR= 0.249, 95% CI=0.063 ± 0.959; P=0.05). On
the other hand, our results showed that a significant correlation was present between metastatic clinicopathological
features and miR-608 (rs4919510) polymorphism (P=0.044).
Conclusion: Our findings reveal that genotypes of rs2292832 and rs4919510 are not associated with
risk of colorectal cancer in Iranian population. Moreover, the CC genotype of rs4919510 contributes
to the metastatic features of the colorectal cancer.
