Quinazoline is an aromatic bicyclic compound exhibiting several pharmaceutical and biological activities.
This study was conducted to investigate the potential wound healing properties of Synthetic Quinazoline
Compound (SQC) on experimental rats. The toxicity of SQC was determined by MTT cell proliferation
assay. The healing effect of SQC was assessed by in vitro wound healing scratch assay on the skin fibroblast
cells (BJ-5ta) and in vivo wound healing experiment of low and high dose of SQC on adult Sprague-Dawley
rats compared with negative (gum acacia) and positive control (Intrasite-gel). Hematoxylin and Eosin (H&E),
Masson’s Trichrome (MT) staining and immunohistochemistry analysis were performed to evaluate the histopathological
alterations and proteins expression of Bax and Hsp70 on the wound tissue after 10 days. In addition,
levels of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase), and malondialdehyde
(MDA) were measured in wound tissue homogenates. The SQC significantly enhanced BJ-5ta cell proliferation
and accelerated the percentage of wound closure, with less scarring, increased fibroblast and collagen
fibers and less inflammatory cells compared with the negative control. The compound also increases endogenous
enzymes and decline lipid peroxidation in wound homogenate.
Keywords: Quinazoline, MTT, wound healing, immunohistochemistry, histopathology, antioxidant activity.
Rights & PermissionsPrintExport