Synthesis of Novel Derivatives of Quinazoline Schiff base Compound Promotes Epithelial Wound Healing

Author(s): Elham Bagheri*, Kamelia Saremi, Fatemeh Hajiaghaalipour, Fadhil Lafta Faraj, Hapipah Mohd Ali, Mahmood Ameen Abdulla, Si Lay Khaing, Nur`Ain Salehen*

Journal Name: Current Pharmaceutical Design

Volume 24 , Issue 13 , 2018

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Abstract:

Quinazoline is an aromatic bicyclic compound exhibiting several pharmaceutical and biological activities. This study was conducted to investigate the potential wound healing properties of Synthetic Quinazoline Compound (SQC) on experimental rats. The toxicity of SQC was determined by MTT cell proliferation assay. The healing effect of SQC was assessed by in vitro wound healing scratch assay on the skin fibroblast cells (BJ-5ta) and in vivo wound healing experiment of low and high dose of SQC on adult Sprague-Dawley rats compared with negative (gum acacia) and positive control (Intrasite-gel). Hematoxylin and Eosin (H&E), Masson’s Trichrome (MT) staining and immunohistochemistry analysis were performed to evaluate the histopathological alterations and proteins expression of Bax and Hsp70 on the wound tissue after 10 days. In addition, levels of antioxidant enzymes (catalase, glutathione peroxidase and superoxide dismutase), and malondialdehyde (MDA) were measured in wound tissue homogenates. The SQC significantly enhanced BJ-5ta cell proliferation and accelerated the percentage of wound closure, with less scarring, increased fibroblast and collagen fibers and less inflammatory cells compared with the negative control. The compound also increases endogenous enzymes and decline lipid peroxidation in wound homogenate.

Keywords: Quinazoline, MTT, wound healing, immunohistochemistry, histopathology, antioxidant activity.

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Article Details

VOLUME: 24
ISSUE: 13
Year: 2018
Page: [1395 - 1404]
Pages: 10
DOI: 10.2174/1381612824666180130124308
Price: $65

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