Association of Thrombospondin-1 (N700S) and Thrombospond in-4 (A387P) Gene Polymorphisms with the Incidence of Acute Myocardial Infarction in Egyptians

Author(s): Noura A. Abdelmonem, Nancy O. Turky, Ingy M. Hashad, Mohamed F. Abdel Rahman, Adel El-Etriby, Mohamed Z. Gad*

Journal Name: Current Pharmaceutical Biotechnology

Volume 18 , Issue 13 , 2017

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Graphical Abstract:


Background: Thrombospondin (TSP) 1 and 4 are extracellular matrix glycoproteins that mediate cell proliferation, platelet aggregation and inflammatory response. Conflicting data addressed the possible contribution of TSP-1 and TSP-4 gene polymorphisms to acute myocardial infarction (AMI).

Objective: Our study aimed to examine the association of TSP-1 (N700S) and TSP-4 (A387P) genetic variants with the incidence of AMI in Egyptians. It also correlated TSP-1 variants to TSP-1 and TNF-α serum concentrations while TSP-4 variants to IL-8 concentration identifying TSPs' contribution to vascular inflammation.

Methods: Genotyping was done in 214 subjects; 114 AMI patients and 100 controls using PCR-RFLP analysis. Serum Tsp-1, TNF-α and IL-8 levels were measured by ELISA assay.

Results: For TSP-4, (GC and CC) genotype distribution and the (C) allele frequency were significantly higher in AMI patients than controls (p = 0.0186), (p = 0.0117) respectively. In contrast, TSP-1 genotypes and allele frequencies showed no significant difference between AMI and controls (p = 0.7124 and p = 0.7201, respectively). Serum TSP-1, TNF-α and IL-8 concentrations were significantly elevated in AMI compared to controls (p = 0.0146, p < 0.0001 and p = 0.0057) respectively. Serum IL-8 levels had a significant difference among TSP-4 genotypes (p= 0.0368), being highest in the mutant C allele. Serum TSP-1 and TNF-α concentrations showed no significant difference among TSP-1 genotypes, but there was a positive correlation between both concentrations in AMI patients (p = 0.0014), (r = 0.4125).

Conclusion: TSP-4 A387P polymorphism, but not TSP-1 polymorphism, is an independent risk factor for AMI in the Egyptians.

Keywords: Acute myocardial infarction, thrombospondin, tumor necrosis factor alpha, interleukin-8, polymorphism, Egyptians, PCR-RFLP.

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Article Details

Year: 2017
Page: [1078 - 1087]
Pages: 10
DOI: 10.2174/1389201019666180115144028
Price: $65

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