In vitro Antioxidant, Antibacterial Activities and HPLC-TOF/MS Analysis of Anvillea radiata (Asteraceae) Extracts

Author(s): Abderrahim Benslama*, Abdenassar Harrar, Fatih Gül, Ibrahim Demirtaş

Journal Name: Current Nutrition & Food Science

Volume 15 , Issue 4 , 2019

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Graphical Abstract:


Background: Algeria has a particular geographical position with a wide band of very varied vegetation including the aromatic and medicinal plants. These plants are able to produce much diversified natural compounds, which can be very useful health care and treatment of diseases.

Objective: This study was carried out to evaluate the antioxidant capacity and antibacterial activity of Anvillea radiata (Asteraceae) extracts, one of the medicinal plants used as food and in folk medicine in Algeria.

Methods: The extraction was carried on the aerial part by methanol and water to give Met.E and Aq.E. The ABTS and DPPH free radicals-scavenging tests are used to evaluate the antioxidant activity of extracts. Moreover, the antibacterial activity was evaluated using disc diffusion method. Finally, the extracts were subject to the phytochemical analysis using the HPLC-TOF/MS equipment.

Results: The Met.E showed the highest amount of total phenolic and flavonoids content (266.12 µg EAG/mg E and 50.83 µg EQ/mg E, respectively). The results of the antioxidant activity revealed that the Met.E showed a good scavenging activity against ABTS and DPPH free radical with EC50=0.067 mg/ml and EC50=0.33 mg/ml, respectively. Moreover, the A. radiata extracts present a highest antibacterial activity against M. luteus NRRL B-4375, B. subtilis NRRL NRS-744 and S. aureus ATCC 25923, with inhibition zone ranging from 18 to 23 mm to Met.E and 12-18 mm to the Aq.E. In addition, the Met.E of A. radiata showed a better MIC against S. aureus, M. luteus and B. subtilis (MIC= 6.25, 25 and 25 µg/ml, respectively). The HPLC-TOF/MS analysis of Met.E reveals the presence of two major compounds, rutin and fumaric acid, when Aq.E contains the syringic acid as a major compound.

Conclusion: The data of our study reported that the Met.E extract of A. radiata had a considerable antioxidant capacity and antibacterial effect, which may be due to their richness by phenolic compounds such as rutin and fumaric acid.

Keywords: Antibacterial activity, antioxidant activity, Anvillea radiata, flavonoids content, HPLC-TOF/MS, phenolic compounds.

Cowan MM. Plants products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
Giliani AH, Rahman A. Trends in ethnopharmacology. J Ethnopharmacol 2005; 100(2): 43-9.
Craig WJ. Health-promoting properties of common herbs. Am J Clin Nutr 1999; 70: 491-9.
Exarchou V, Nenadis M, Tsimidou IP, Gerothanassis A, Troganis D, Boskou A. Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage, and Summer savory. J Agric Food Chem 2002; 50: 5294-9.
Halliwell B. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. Am J Med 1991; 91: S14-22.
Afanas’ev I. Signaling and damaging functions of free radicals in aging-free radical theory, hormesis, and TOR. Aging Dis 2010; 1(2): 75-88.
Shahidi F, Zhong Y. Measurement of antioxidant activity. J Funct Foods 2015; 18: 757-81.
Taylor PW, Stapleton PD, Paul-Luzio J. New ways to treat bacterial infections. Drug Discov Today 2002; 7: 1086-91.
Morel J, Lescoat G, Cogrel P, et al. Antioxidant and iron chelating activities of the flavonoids catechin, quercetin and diosmetin on iron loaded rat hepatocyte cultures. Biochem Pharmacol 1993; 7(45): 13-9.
Hanasaki YS, Ogawa S, Fukui D. The correlation between active oxygens scavenging and effects of flavonoids. Free Radic Biol Med 1994; 16: 845-50.
Bouallala M, Bradai L, Abid M. Diversité et utilisation des plantes spontanées du Sahara septentrional algérien dans la pharmacopée saharienne. Cas de la région du Souf. Revue ElWahat pour les Recherches et les Etudes 2014; 7(2): 18-26.
El Rhaffari L. Zaid. Pratique de la phytothérapie dans le sudest du Maroc (Tafilalet): Un savoir empirique pour une pharmacopée rénovée 2002; 293-318.
Motamed SM, Naghibi F. Antioxidant activity of some edible plants of the Turkmen Sahra region in northern in Iran. Food Chem 2010; 119: 1637-42.
Li HB, Cheng KW, Wong CC, Fan KW, Chen FD, Jiang YS. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem 2007; 102: 771-6.
Bahorun T, Gressier B, Trotin F, et al. Oxygen species scavenging activity of phenolic extract from howthorn fresh plant organs and pharmaceutical preparation. Arzneimittelforschung 1996; 46: 1086-94.
Huang B, Ke HB, He JS, Ban XQ, Zeng H, Wang YW. Extracts of Halenia elliptica exhibit antioxidant properties in vitro and in vivo. Food Chem Toxicol 2011; 49: 185-90.
Benslama A, Harrar A. Free radicals scavenging activity and reducing power of two Algerian Sahara medicinal plants extracts. Inter J Herb Med 2016; 4(6): 158-61.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016; 6: 71-9.
Gulluce M, Sokmen M, Daferera D, et al. In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extract of herbal parts and callus cultures of Satureja hortensis L. J Agric Food Chem 2003; 51(14): 3958-65.
Abay G, Altun M, Koldas S, Tufekci AR, Demirtas I. Determination of antiproliferative activities of volatile contents and HPLC profiles of Dicranum scoparium (Dicranaceae, Bryophyta). Comb Chem High Throughput Screen 2015; 18: 453-563.
Zhou K, Yu L. Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT-Food Sci Technol 2004; 37: 717-21.
Do QD, Angkawijaya AE, Tran-Nguyen PL, et al. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. J Food Anal 2014; 22: 296-302.
Meneses NGT, Martins S, Teixeira JA, Mussatto SI. Influence of extraction solvents on the recovery of antioxidant phenolic compounds from brewer’s spent grains. Separ Purif Tech 2013; 108: 152-8.
Ghedadba N, Hambaba L, Aberkane MC, Oueld-Mokhtar SM, Fercha N, Bousselsela H. Évaluation de l’activité hémostatique in vitro de l’extrait aqueux des feuilles de Marrubium vulgare L. Algerian J Nat Products 2014; 2(2): 64-74.
Huang D, Ou B, Prior L. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005; 53: 1841-56.
Craft BD, Kerrihard AL, Amarowicz R, Pegg RB. Phenol-based antioxidants and the in vitro methods used for their assessment. Compr Rev Food Sci Food Saf 2012; 11: 148-73.
Vukics V, Kery A, Bonn GK, Guttman A. Major flavonoid components of heartsease (Viola tricolor L.) and their antioxidant activities. Anal Bioanal Chem 2008; 390: 1917-25.
Nur Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013; 21(2): 143-52.
Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63: 1035-42.
Hu JP, Calomme M, Lasure A, et al. Structure-activity relationship of flavonoids with superoxide scavenging activity. Biol Trace Elem Res 1995; 47: 327-31.
Zheng W, Wang SY. Antioxidant activity and phenolic compounds in selected herbs. J Agric Food Chem 2001; 49: 5165-70.
Maksimović Z, Malenčić D, Kovačević N. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresour Technol 2005; 96: 873-7.
Moteriya P, Ram J, Moradiya R, Chanda S. In vitro free radical scavenging and antimicrobial activity of Cyamopsis tetragonoloba L. J Pharmacog Phytochem 2015; 4(2): 102-6.
Penna C, Marino S, Vivot E, Munoz M, Cruanes J, Ferraro G. Antimicrobial activity of Argentine plants used in the treatment of infectious diseases. J Ethnopharmacol 2001; 77: 37-40.
Rios JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol 2005; 100: 80-4.
Cushnie PT, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005; 26: 343-56.
Oyaizu M, Fujimoto Y, Ogihara H, Sekimoto K, Naruse A, Naruse U. Antioxidative and antimicrobial activities extracts from several utility plants. J Food Process Preserv 2003; 29: 33-8.
Ahmed I, Aqil F. In vitro efficacy of bioactive extracts of 15 medicinal plants against ESbL-producing multidrug-resistant enteric bacteria. Microbiol Res 2007; 162: 264-75.
Jigna P, Rathish N, Sumitra C. Preliminary screening of some folklore plants from Western India for potential antimicrobial activity. Int J Pharmacol 2005; 37: 408-13.
He CL, Fu BD, Shen HQ, Jiang XL, Wei XB. Fumaric acid, an antibacterial component of Aleo vera L. Afr J Biotechnol 2015; 10(15): 2973-7.
Baati T, Horcajada P, Gref R, Couvreur P, Serre C. Quantification of fumaric acid in liver, spleen and urine by high-performance liquid chromatography coupled to photodiode-array detection. J Pharm Biomed Anal 2011; 56: 758-62.
Al-Dhabi NA, Arasu MV, Park CH, Park SU. An up-to-date review of rutin and its biological and pharmaco-logical activities. EXCLI J 2015; 14: 59-63.
Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-52.

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Article Details

Year: 2019
Published on: 28 June, 2019
Page: [376 - 383]
Pages: 8
DOI: 10.2174/1573401314666171204161538
Price: $65

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