Background: Recently, the development of a safe bioactive material with antioxidant properties,
which can improve healing activity are focusing. Biosurfactants are very famous for their antimicrobial
and free radical scavenging activities. Thereof, the main aim of the present study was to investigate
the antioxidant and wound healing activity of the lipopeptide biosurfactant (LBS) produced by
Acinetobacter junii B6. DPPH radical scavenging activities and FRAP assays were used to measure the
Methods: For evaluation of the wound healing activity, 36 rats (previously wounded in depilated thoracic
region) were randomly distributed into six groups and chromatic, wound contraction, and histopathological
feature were examined. The assessment levels of reactive oxygen species (ROS) after
LBS exposure were determined using malondialdehyde (MDA), hydrogen peroxide (H2O2), and glutathione
(GSH) assay kits.
Results: DPPH assay showed notable scavenging activities at the corresponding concentrations with
IC50 value of 0.7 mg/ml. The reductive potency of the LBS showed lower performance at low concentration,
while exhibited a remarkable increase at higher concentration. The best histopathological remission
was achieved following treatment by 5 mg/ml of the LBS. Scar wounds at day 13 showed the
lowest lesion sizes, increased re-epithelialization, hair follicle detection, and decreased amounts of
neutrophilic inflammation, immaturity of the wound bed, erythema, edema, capillary, and retention of
Conclusion: Results from MDA, H2O2, and GSH levels of the treated sample confirmed the scavenging
property of the bacterial derived LBS through ROS. It could be concluded that the pharmaceutical
formula encourages the wound healing because of its notable antioxidant capacity.
Highlights: • DPPH and FRAP assays showed notable scavenging activity.
• MDA, H2O2, and GSH; confirmed the scavenging property of the derived biosurfactant through ROS.
• Synthesized formula encourages the healing of the wound because of its antioxidant capacity.