Title:Application of Gold Nanorods for the Evaluation of Antioxidant Activity
VOLUME: 14 ISSUE: 6
Author(s):Caiyun Jiang, Yuan Liu, Xiaoyuan Ma, Hong-zhen Lian* and Yuping Wang*
Affiliation:State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, State Key Laboratory of Analytical Chemistry for Life Science, Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023
Keywords:Gold nanorods, longitudinal surface plasmon resonance, antioxidation activity, scented tea, ascorbic acid, rate of
oxidation.
Abstract:Background: Excessive active oxygen will suppress the antioxidant defense system, resulting
in a series of negative effects. Antioxidant substances in the diet can protect important biological
molecules by mitigating the harm caused by oxidative stress and reducing the occurrence of chronic
diseases related to aging. Therefore, simple, reliable and rapid detection methods for screening potential
antioxidants in the diet have become very important.
Methods: 1.0 g scented tea was added into 25 mL boiling water, and the extracting solution was filtered
through filter paper by normal funnel after 10 min stirring extraction. After cooled to room temperature,
the filtrate was diluted with water. Then, 100 µL of the scented tea extract was added into 300 µL of
GNR solution, and water was added until the total volume was 900 µL. Subsequently, 900 µL of CTAB
solution (0.2 mol/L) and 10 µL of hydrochloric acid (3 mol/L) were added successively. After mixing,
800 µL of H2O2 solution (1.26 mol/L) was added for the reaction. The variation of the GNR absorption
spectrum with time was measured to determine the inhibition of oxidation and IC50.
Results: The rate of oxidation of GNRs was reduced by the addition of antioxidant substance, such as
scented teas. The stronger the antioxidant activity was, the lower the rate of GNR oxidation and the
smaller the change in the longitudinal absorption peak. The blue-shift of the longitudinal absorption
wavelength was monitored by ultraviolet-visible spectroscopy, enabling evaluation of the antioxidant
activity of scented teas. The IC50 values of AA, Lophatherum gracile Brongn, rose, lavender, hawthorn
and lotus leaf were 0.0088, 0.0338, 0.0540, 0.0907, 0.2383 and 0.2533 mg/mL, respectively.
Conclusion: The relative antioxidant activities from strong to weak for these five scented teas were as
follows: Lophatherum gracile Brongn > rose > lavender > hawthorn > lotus leaf. The order was in good
accordance with that obtained by the conventional DPPH method. This method is simple and easy to
operate, interference-free, and provides a promising solution for filed screening of antioxidant activity
of natural products.
