Insulin-like Effects of Mt. Fuji Subsoil Water which Conta ins Vanadium on Cultured Cells: Insight from Japan

Author(s): Kenji Sorimachi*, Yuki Ohmori, Takehisa Matsukawa, Kazuhito Yokoyama, Shuji Ohhira

Journal Name: Current Traditional Medicine

Volume 3 , Issue 3 , 2017

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Graphical Abstract:


Background: It was reported that vanadium compounds possess insulin-like activity, although suitable vanadium compounds have not yet developed as anti-diabetes mellitus drugs.

Objective: As Mt. Fuji subsoil water contains high levels of vanadium, this study aimed to assess whether it showed insulin-like effects.

Method: Insulin-like effects of vanadium ions have been investigated in cultured cells, using rat fibroblasts (Py-3Y1-S2), human esophageal cancer cells (TE-13), and other established cell lines. To evaluate insulin-like effects, glucose consumption in the cultured media and cell growth based on cellular protein content were measured, and cytotoxic effects on cells were assessed.

Results: The vanadium concentration in the commercially available Mt. Fuji subsoil water ranged from 56–144 ppb, and the highest concentration (144.2 ± 1.2 ppb) was observed in Healthy Vana Water. The vanadium concentration in other water samples derived from wells, ravine rivers, and cities ranged from 0.5 to 27 ppb, and a comparatively high vanadium concentration (27.1 ± 0.0 ppb) was observed in the well water at Kayayama-Machi, which is close to Mt. Fuji. The largest insulin-like effect on glucose consumption was observed in Healthy Vana Water among the various water samples tested. The effects of vanadium at low concentrations of 200-500 µg/l seemed to accelerate cell growth, while high concentrations of vanadium (> 500 µg/l) had a cytotoxic effect.

Conclusion: Mt. Fuji subsoil water possesses higher insulin-like activity than other water samples derived from wells, ravine rivers, and cities, suggesting a potential future role in diabetes mellitus therapy.

Keywords: Insulin-like effects, diabetes, vanadium, Mt. Fuji subsoil water, glucose consumption, cell growth, cytotoxicity.

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Article Details

Year: 2017
Published on: 16 October, 2017
Page: [178 - 189]
Pages: 12
DOI: 10.2174/2215083803666171017101043
Price: $25

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