Background and Methods: Ratiometric fluorescent probes can provide more
precise measurement results than intensity-based fluorescent probes. Carbon dots are
convenient platforms to construct ratiometric fluorescent probes. Carbon dots-based ratiometric
fluorescent probes can be constructed by carbon dots and other fluorophores’
simple mixing or by connecting them via covalent bonds or non-covalent bonds. Otherwise,
dual-emission carbon dots can also be taken as the ratiometric fluorescent probes.
Carbon dots are reference signals, energy donors, response signals and the double emission
matrixes in ratiometric probes.
Conclusion: The development of carbon dots-based ratiometric fluorescent probes in recent
five years is reviewed in this study which emphasizes their photoluminscene properties,
detection mechanisms and applications.