Introduction: Parathyroid Hormone (PTH) is an effective therapeutic agent for osteoporosis,
but the treatment requires long-term daily injections. Oral gene delivery is a less invasive alternative
to daily injections.
Objective: The aim of this study is to investigate the effect of orally administered nonionic polymeric
micelles of plasmid cDNA containing human cytomegalovirus promoter (PCMV)-PTH (1-34) plus
EDTA on body mineral density and bone microstructure in ovariectomized rats.
Material and Methods: A total of 27 Spraque-Dawley female rats were subjected to a bilateral ovariectomy.
One month following the ovariectomy, they were randomly assigned to three groups: (1) (PCMVPTH
(1-34) cDNA in polyethylene oxide-polypropylene oxide-polyethylene oxide (PEO-PPO-PEO) polymeric
micelle formations plus EDTA); (2) PCMV-PTH (1-34) cDNA and (3) drinking water. The
treatment was administered by oral gavage on day 1, 2, 7, 14, and 21 at 8-hour intervals. Body mineral
density, bone volume fraction, and trabecular thickness of the lumbar spine and femoral neck were examined
with peripheral quantitative computed tomography at pre- and post-intervention (3 months after
the start of the intervention) and analyzed using two-way repeated measures analysis of variance.
Results: Results showed that bone mineral density, bone volume fraction and trabecular thickness
were significantly increased in Group 1 over time, compared with those in Group 2 and Group 3.
Conclusion: In conclusion, significantly improved bone mineral density and bone microstructure were observed
in ovariectomized rats treated with PTH (1-34) cDNA delivered by nonionic polymeric micelles.