Background and Objective: Glycolytic activity during Crabtree effect is similar to that in
tumor cells. Research regarding Crabtree effect is very much crucial.
Methods: The mechanism of metabolic activities in glycolysis pathway and oxidative phosphorylation
pathway in regards to Crabtree effect in Saccharomyces cerevisiae was studied in this paper. We also
explored the effects of hexose phosphates in the activities of respiratory chain complexes (III and IV)
in inhibition of respiration. Besides, the enhancement of fermentation in response to excess glucose
concentration was studied. We discussed the significance of Crabtree effect in mammalian cancer in
terms of Crabtree effect in a Crabtree positive organism, as it is similar to cancer metabolism in mammalian
cells. We developed an in silico model of Crabtree effect.
Results: A comparative study was performed with laboratory experiments regarding inhibitory role of
fructose 1,6-bisphosphate on metabolic respiration. The model was simulated for different concentration
levels of glucose and hexose phosphates using COPASI and SNOOPY tools.
Conclusion: We have shown that a hike in glucose concentration increases ethanol concentration and
leads glycolytic activity towards fermentation. This phenomenon occurs during Crabtree effect.
Keywords: Metabolism, glycolysis, oxidative phosphorylation, Crabtree, in silico modeling, tumor cells.
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