Title:Glycan Phosphorylases in Multi-Enzyme Synthetic Processes
VOLUME: 24 ISSUE: 8
Author(s):Giulia Pergolizzi, Sakonwan Kuhaudomlarp, Eeshan Kalita and Robert A. Field*
Affiliation:Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH
Keywords:Phosphorylase, disaccharide, α-glucan, cellodextrin, high-value products, biofuel.
Abstract:Glycoside phosphorylases catalyse the reversible synthesis of glycosidic bonds by glycosylation
with concomitant release of inorganic phosphate. The equilibrium position of such reactions
can render them of limited synthetic utility, unless coupled with a secondary enzymatic step
where the reaction lies heavily in favour of product. This article surveys recent works on the combined
use of glycan phosphorylases with other enzymes to achieve synthetically useful processes.
