Simultaneous Determination of Kirenol, Rosmarinic Acid and Caffeic Acid in Rat Plasma and Pharmacokinetic Study After Oral Administration of the Extract of Manxingshizhen Preparation by UPLC-MS/MS

Author(s): Shuo Sun, Xue Zhang, Linda Luo, Ping Wang, Mengxuan Bai, Jia Yu*, Xingjie Guo*

Journal Name: Current Pharmaceutical Analysis

Volume 15 , Issue 1 , 2019

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Graphical Abstract:


Introduction: A rapid, sensitive and convenient ultra-performance liquid chromatography with tandem mass spectrometric detection (UPLC-MS/MS) method has been validated and applied to the simultaneous determination of kirenol, rosmarinic acid and caffeic acid after oral administration of the extract of Manxingshizhen preparation in rat plasma.

Materials and Methods: Puerarin was selected as the internal standard (IS). The plasma sample preparation was pretreated by liquid-liquid extraction of the mixture with ethyl acetate. All analytes were simultaneously detected in multiple reaction monitoring (MRM) mode via both the positive electrospray ionization (ESI+) and negative electrospray ionization (ESI). In the experiment, all calibration curves revealed good linearity (r > 0.999). The LLOQ were between 0.80-2.00 ng/mL, respectively.

Besides, the intra-day and inter-day precision ranged from 6.4 to 13.8%, respectively. Moreover, the accuracy was within - 11.4% and 12.8% for all the QC levels of all analytes. The extraction recoveries of the analytes and IS in plasma at three concentration levels ranged from 88.5 to 103.2%, moreover, the matrix effects of all the analytes and the IS were found to be satisfied with the acceptable range of 89.8%-101.7%. Meanwhile, the RSD values of stability met the requirement of not more than 15%. Furthermore, the pharmacokinetic parameters of three compounds were analyzed using concentrationtime profiles.

Conclusion and Results: Plasma concentrations of the three compounds were determined up to 24 h after oral administration, and their pharmacokinetic parameters were in agreement with previous studies. The validated method was successfully applied in a pharmacokinetic study in rat plasma after oral administration of Manxingshizhen preparation.

Keywords: Manxingshizhen preparation, UPLC-MS/MS, rat plasma, pharmacokinetic study, kirenol, rosmarinic acid, caffeic acid.

Liu, D.; Jia, X.B. Analysis and discussion about current development of relevant studies on traditional Chinese medicine components. China J. Chinese Materia. Medica., 2014, 39(2), 171-174.
Du, L.Y.; Guo, J.M.; Qian, D.W.; Shang, E.X.; Jiang, S.; Liu, P.; Su, S.L.; Zhang, F.; Duan, J.A.; Xu, J.; Zhao, M. Simultaneous determination of seven active ingredients in rat plasma by UPLC-MS/MS and application in pharmacokinetic studies after oral administration of scutellaria coptis herb couple. J. Medic. Chem. Res., 2015, 24(3), 1289-1297.
Shao, Y.P.; Zhang, W.; Tong, L.; Huang, J.Y.; Li, D.X.; Nie, W.; Zhu, Y.; Li, Y.F.; Lu, T. Simultaneous determination of eight bioactive components of Qishen Yiqi Dripping Pills in rat plasma using UFLC-MS/MS and its application to a pharmacokinetic study, J. Biomedical Chromatography., 2017.
Jiang, Z.; Yu, Q.H.; Cheng, Y.; Guo, X.J. Simultaneous quantification of eight major constituents in HerbaSiegesbeckiae by liquid chromatography coupled with electrospray ionization time-of-flight tandem mass spectrometry. J. Pharmaceut. Biomed. Anal., 2011, 55(3), 452.
Lu, Y.; Xiao, J.; Wu, Z.W.; Wang, Z.M.; Hu, J.; Fu, H.Z.; Chen, Y.Y.; Qian, R.Q. Kirenol exerts a potent anti-arthritic effect in collagen-induced arthritis by modifying the T cells balance. J. Phyto. Medi., 2012, 19(10), 882-889.
Jezyna, I.; Skała, W.E.; Olszewska, M.A.; Kiss, A.K.; Balcerczak, E.; Wysoki’nska, H.; Kicel, A. The identification and quantitative determination of rosmarinic acid and salvianolic acid B in hairy root cultures of Dracocephalum forrestii W.W. Smith. J. Industrial Crops Products., 2016, 91, 125-131.
Wang, H.; Provan, G.J.; Helliwell, K. Determination of rosmarinic acid and caffeic acid in aromatic herbs by HPLC. J. Food Chem., 2004, 87(2), 307-311.
Maurya, D.K.; Devasagayam, T.P. Antioxidant and prooxidant nature of hydroxycinnamic acid derivatives ferulic and caffeic acids. Food Chem. Toxicol., 2010, 48(12), 3369-3373.
Sun-Waterhouse, D.; Zhou, J.; Miskelly, G.M.; Wibisono, R.; Wadhwa, S.S. Stability of encapsulated olive oil in the presence of caffeic acid. J. Food Chemi., 2011, 126(3), 1049-1056.
Liu, Z.; Chou, G.; Wang, Z. Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography. J. China J. Chinese Materia. Medica., 2010, 35(6), 729-731.
Li, N.; Liu, C.H.; Mi, S.Q.; Wang, N.S.; Zheng, X.; Li, Y.Y.; Huang, X.T.; He, S.L.; Chen, H.; Xu, X.H. Simultaneous determination of oleanolic acid, p-coumaric acid, ferulic acid, kaemperol and quercetin in rat plasma by LC-MS-MS and application to a pharmacokinetic study of oldenlandia diffusa extract in rats. J. Chromatogr. Sci., 2012, 50(10), 885-892.
Basu, S.; Patel, V.B.; Jana, S.; Patel, H. Liquid chromatography tandem mass spectrometry method (LC-MS/MS) for simultaneous determination of piperine, cinnamic acid and gallic acid in rat plasma using a polarity switch technique. J. Anal. Methods, 2013, 5(4), 967-976.
Zhang, B.; Chen, J.G.; Jiang, Q.C.; Wang, X.F.; Lu, Y.; Gong, L.; Chen, D. Simultaneous determination of three active components in rat plasma by UPLC-MS/MS: Application to pharmacokinetic study after oral administration of Herba Sarcandrae extract. J. Biomed. Chromatogr., 2017, 31, 3834.
Pang, Q.; Tian, Y.Y.; Mi, J.P.; Wang, J.; Xu, Y.J. Simultaneous determination and pharmacokinetic study of eight components in rat plasma by UHPLC-MS/MS after oral administration of Hypericum japonicum Thunb extract. J. Pharmaceut. Biomed. Anal., 2016, 118, 118-228.
Gao, J.W.; Qiu, Y.; Chen, J.M.; Mu, S.X.; Sun, L.X. Simultaneous determination of nineteen major active compounds in Qiangshen tablet by UPLC-ESI-MS/MS. J. Pharmaceut. Biomed. Anal., 2016, 128, 519-527.
Tian, Y.Y.; Li, Q.Q.; Zhou, X.P.; Pang, Q.; Xu, Y.J.A. UHPLC-MS/MS method for simultaneous determination of twelve constituents from Erigeron breviscapus extract in rat plasma: Application to a pharmacokinetic study. J. Chromatogr. B., 2017, 1046, 1-12.

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Article Details

Year: 2019
Published on: 28 November, 2018
Page: [74 - 81]
Pages: 8
DOI: 10.2174/1573412913666170808100104
Price: $65

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