In Vitro Effects of Nickel on Healthy Non-Allergic Peripheral Blood Mononuclear Cells. The Role of Red Grape Polyphenols

Author(s): Thea Magrone*, Rosaria Salvatore, Anna Spagnoletta, Manrico Magrone, Matteo Antonio Russo, Emilio Jirillo

Journal Name: Endocrine, Metabolic & Immune Disorders - Drug Targets
Formerly Current Drug Targets - Immune, Endocrine & Metabolic Disorders

Volume 17 , Issue 2 , 2017

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Graphical Abstract:


Background: Nickel (Ni) is widely distributed in the environment and continuous exposure to this metal may lead to pathological manifestations, such as the human allergic contact dermatitis.

Methods: The in vitro effects of Ni on human healthy non allergic peripheral blood mononuclear cells (PBMCs) in the absence or presence of red grape polyphenols have been evaluated. In the culture supernatants, levels of cytokines have been determined by ELISA, while nitric oxide (NO) concentration has been evaluated by a colorimetric procedure.

Results: Ni per se did not affect release of interferon-γ, interleukin (IL)-4 and IL-10. Instead, this metal dramatically reduced production of IL-17 which was restored by the supplementation of polyphenols. Finally, while Ni significantly increased generation of NO, polyphenols reduced production of this compound.

Conclusion: Taken together, all these data may indicate a preventative role of polyphenols against Ni exposure in non allergic to Ni individuals, also confirming the immunomodulating role of these natural products. The interaction Ni/polyphenols/lipopolysaccharides will also be discussed.

Keywords: Lipopolysaccharides, nitric oxide, non-allergic, nickel individuals, peripheral blood mononuclear cells, polyphenols.

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Article Details

Year: 2017
Published on: 11 September, 2017
Page: [166 - 173]
Pages: 8
DOI: 10.2174/1871530317666170713145350
Price: $65

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