The Potential Clinical Application of Mesenchymal Stem Cells from the Dental Pulp (DPSCs) for Bone Regeneration
Pp. 21-70 (50)
Ana Rita Caseiro, Rui Alvites, Sílvia Santos Pedrosa, José Miguel Campos, Inês Leal Reis, José Domingos Santos, Carla Mendonça, Luís Miguel Atayde and Ana Colette Maurício
The skeleton has the vital role of providing support and protection to all
body organs, as well as to function as storage system for ions and other components
essential to its homeostasis, also presenting essential function in the movement and
posture of the individual. Bone fractures are a fairly common situation affecting
individuals of all ages, but gain importance when concomitant pathologies are present
or when the bone lesions’ extension exceeds the tissues’ intrinsic healing capabilities.
As such, biomedical research has invested in unveiling adequate therapies to aid in
those cases. The tissue engineering field has therefore evolved in the direction of
developing biomaterials and scaffolds to structure, support and promote bone ingrowth,
and in developing strategies to optimize these biomaterials in vivo performance, by
including cell-based therapies and growth factors. Herein, we discuss one promising
strategy for the optimization of these hybrid systems, through the association of
biomaterials to a specific source of mesenchymal stem cells: the dental pulp stem cells.
Dental pulp stem cells can be found in individuals of any age, and can be easily
isolated from deciduous and definitive teeth, expanded and cryopreserved for further use. These cells are capable of differentiating towards multiple lineages, presenting
great potential for osteo-differentiation. Dental pulp stem cells have been demonstrated
to incorporate diverse biomaterial systems and promote mineral deposition both in vitro
and in vivo, aiming at the reconstruction of osseous defects, in either experimental or
clinical situations. The mesenchymal stem cells from the dental pulp can also be found
and isolated from many species other than humans, granting them potential to be
implemented not only in human medicine but also in veterinary care practices, and in
regenerative strategies for other organs and tissues, such as dental reconstruction and
nervous system regeneration.
Adult teeth, Animal models, Biomaterials, Bone regeneration, Cell
isolation, Cell sources, Ceramic biomaterials, Deciduous teeth, Dental cells,
Dental pulp, Dental regeneration, Differentiation, Growth factors, In vitro, In
vivo, Mesenchymal stem cells, Nerve regeneration, Stem cells, Tissue
Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Rua de Jorge Viterbo Ferreira, nº 228, 4050-313 Porto, Portugal.