Abstract
Introduction: This study reports the uses of Pterospermum acerifolium leaf extract (PALE) as a novel reducing agent for the synthesis of silver nanoparticles.
Materials and Methods: These PALE silver nanoparticles (PaAgNPs) characterized by UVVis spectrophotometer and HR-TEM were spherical in shape and their sizes range from 10- 20 nm. FTIR spectrum revealed the presence of polycyclic compounds similar to betulinic acid which acts as capping and stabilizing agent to PaAgNPs. XRD analysis showed crystalline nature of PaAgNPs. Stability of PaAgNPs was checked by Zeta potential. Antineoplastic effects of PaAgNPs on HepG2 cells were monitored by MTT assay, DAPI, and AO-EB staining. Mortality rate of up to 94.6 % was observed at 250 µg/ml with IC50 value 4.76 ± 1.1µg/ml after 48 h however negligible effect was detected on normal human embryonic kidney (HEK 293) cells.
Result: Fluorescence in images revealed shrinkage, condensed nuclei, fragmentation and the formation of apoptotic bodies in PaAgNPs treated HepG2 cells. The phytofabricated PaAgNPs also showed antimicrobial activity using well diffusion assay against S. aureus, B. subtilis, E. coli, S. flexneri, and C.albicans.
Conclusion: The investigation unveils that phytofabricated PaAgNPs could be exploited as antineoplastic and antimicrobial agents.
Keywords: PaAgNPs, spectroscopy, FTIR, HR-TEM, MTT, antimicrobials.
Graphical Abstract
Related Books
Nanoscale Field Effect Transistors: Emerging Applications
Nanoelectronics Devices: Design, Materials, and Applications Part II
Nanoelectronics Devices: Design, Materials, and Applications (Part I)
Role of Nanotechnology in Cancer Therapy
Synthesis and Applications of Semiconductor Nanostructures
Pathways to Green Nanomaterials: Plants as Raw Materials, Reducing Agents and Hosts
Applications of Nanomaterials in Medical Procedures and Treatments
Synthesis of Nanomaterials
Nanobiotechnology: Principles and Applications
Bio-Inspired Nanotechnology
9
2