Background: Traditionally plants are being used as an important source of medicines
since ancient era. Cucurbit seeds have been used as nutritious snacks as they are rich in
phytonutrients and have a wide arena of ethnopharmacological relevance.
Objective: Present research study was designed to identify the phytochemicals in n-hexane
seed extracts of Cucurbita pepo (CP), Cucumis melo (CM), Cucumis sativus (CS) belonging to
family Cucurbitaceae by hyphenated techniques such as Gas Chromatography – Mass Spectroscopy
Method: Soxhlet extraction of Cucurbita pepo , Cucumis melo, Cucumis sativus seeds was
done using n-hexane as solvent and GCMS analysis was performed using Joel, USA with
model of Accu Time of Flight GCV.
Result: The components were identified by matching mass spectra with mass spectrum libraries.
There were six, eight and nine different compounds analysed from CP, CM and CS
extracts respectively. The components present were Palmitic acid; 17-Octadecynoic acid;
Z-7-Hexadecenal; Squalene; 9,12-Octadecadienoic acid (Z,Z)-; γ-Tocopherol; 17-
Octadecynoic acid; 1-Heptatriacotanol; Cholest-22-ene-21-ol-3,5-dehydro-6-methoxy-, pivalate;
acid, 2-(acetyloxy)-1-[(acetyloxy)methyl]ethyl ester,(Z,Z,Z)-; 1-Heptatriacotanol; Ergosta-
7,22-dien-3-ol,(3β,5α,22E)-; Stigmaterol; D:C-Friedours-7-en-3-one in CP, CM and
CS seeds extracts respectively.
Conclusion: From the results obtained it is concluded that Cucurbita pepo , Cucumis melo,
and Cucumis sativus contain various phytochemicals, justifying their use for various human