Background: Acidic polysaccharides usually possess favorable bioactivities, and some of
them are applied in medicines and dietary supplements together. However, it is a challenge to quantify
these polysaccharides simultaneously.
Methods: A method to Quantify Hyaluronic Acid (HA), Chondroitin Sulfate (CS) and a nonglycosaminoglycan
acidic polysaccharide named AGSP was established by determining the contents of
their disaccharides produced from the acid hydrolysis using hydrophilic interaction chromatographytandem
triple quadrupole mass spectrometry in the positive mode.
Results: This method had excellent linearities in a concentration range of 0.02~2.00 mg/mL for these
standard acidic polysaccharides, the recoveries were all 95%~112%, and relative standard deviations
(RSDs) were less than 5%. Limit of Detection (LOD) and Limit of Quantification (LOQ) ranged from
0.1 µg/mL to 4.1 µg/mL. It could be inferred that the demonstrated method was feasible to quantitatively
detect uronic acid-containing polysaccharides.
Conclusion: This method could be a useful tool for quality control of medicines and dietary supplements
containing these acidic polysaccharides.