Background: Angiogenesis is critical for the growth of tumor by supplying nutrients and
oxygen that exacerbates the metastasis and progression of cancer. Noninvasive imaging of angiogenesis
during the tumor therapeutic processes may provide novel opportunities for image-guided tumor
Objective: Here, we want to develop a mouse animal model for assessing cancer progression and angiogenesis
in the same individuals by molecular imaging.
Methods: Breast cancer model was developed with mouse breast cancer cell line 4T1 carrying a reporter
system encoding a triple fusion (TF) reporter gene consisting of renilla luciferase (Rluc), red
fluorescent protein (RFP) and herpes simplex virus truncated thymidine kinase (HSV-ttk) in transgenic
mice, which expressed firefly luciferase (Fluc) under the promoter of vascular endothelial growth factor
receptor 2 (Vegfr2-luc). The mice were subsequently treated with ganciclovir (GCV) and the tumor
angiogenesis was tracked by Fluc imaging and the growth status of tumor was monitored by imaging
of Rluc simultaneously.
Conclusion: Overall, this traceable breast cancer model can simultaneously image the tumor growth
and angiogenesis in single individual, which may facilitate a better understanding the mechanisms of
angiogenesis in the progression and regression of tumor.